Schoofs A, Odds F C, Colebunders R, Ieven M, Goossens H
Department of Dermatology, University Hospital of Antwerp, Edegem, Belgium.
Eur J Clin Microbiol Infect Dis. 1997 Apr;16(4):296-300. doi: 10.1007/BF01695634.
During a study of oral rinses of 130 HIV-infected individuals, both typical and atypical Candida albicans colonies were isolated from ten patients on a yeast differential medium. Typical Candida albicans colonies were light green; atypical colonies were dark green. Both types of colonies were germ tube-positive and produced chlamydospores. However, DNA fingerprinting of the atypical isolates with the Ca3 Candida albicans-specific probe showed that they belonged to the recently described species Candida dubliniensis. Candida dubliniensis colonies could also be differentiated from Candida albicans colonies on isolation plates by the absence of fluorescence of colonies on methyl blue-Sabouraud agar under Wood's light. Among other phenotypic characteristics, only the absence of intracellular beta-glucosidase activity reliably distinguished Candida albicans from Candida dubliniensis. Candida dubliniensis may be underreported in clinical samples because most currently used isolation and identification methods fail to recognize this yeast.
在一项对130名HIV感染者口腔含漱液的研究中，在酵母鉴别培养基上，从10名患者中分离出了典型和非典型白色念珠菌菌落。典型的白色念珠菌菌落呈浅绿色；非典型菌落呈深绿色。两种菌落均为芽管阳性且产生厚壁孢子。然而，用Ca3白色念珠菌特异性探针进行的非典型分离株DNA指纹分析表明，它们属于最近描述的都柏林念珠菌物种。在分离平板上，都柏林念珠菌菌落与白色念珠菌菌落也可通过在伍德灯下甲基蓝-沙氏琼脂上菌落无荧光来区分。在其他表型特征中，只有细胞内β-葡萄糖苷酶活性的缺乏能可靠地将白色念珠菌与都柏林念珠菌区分开来。都柏林念珠菌在临床样本中可能报告不足，因为目前大多数常用的分离和鉴定方法无法识别这种酵母。
