Boerlin P, Boerlin-Petzold F, Durussel C, Addo M, Pagani J L, Chave J P, Bille J
Institute of Microbiology, University Hospital, CHUV-BH19SUD, Lausanne, Switzerland.
J Clin Microbiol. 1995 May;33(5):1129-35. doi: 10.1128/jcm.33.5.1129-1135.1995.
Twenty-one chlamydospore-forming and germ tube-positive Candida albicans clinical isolates from 15 human immunodeficiency virus (HIV)-positive and 3 HIV-negative patients were examined by two different genetic methods. Multilocus enzyme electrophoresis and hybridization with the C. albicans-specific Ca3 probe showed that such isolates can be split into two genetically distinct groups that can be clearly distinguished. One group mainly contained strains with atypical sugar assimilation patterns and could be distinguished from the other group by the absence of intracellular beta-glucosidase activity. All 13 strains belonging to this group were isolated from the oral cavities of asymptomatic HIV-positive drug users and may be less pathogenic than the eight strains from the other group isolated either from HIV-positive patients with oropharyngeal candidiasis or from HIV-negative patients with invasive candidiasis.
采用两种不同的基因方法，对来自15名人类免疫缺陷病毒（HIV）阳性患者和3名HIV阴性患者的21株形成厚垣孢子且芽管阳性的白色念珠菌临床分离株进行了检测。多位点酶电泳以及与白色念珠菌特异性Ca3探针杂交结果表明，这些分离株可分为两个遗传上截然不同且能清晰区分的组。一组主要包含具有非典型糖同化模式的菌株，且通过缺乏细胞内β-葡萄糖苷酶活性可与另一组区分开来。该组的所有13株菌株均从无症状HIV阳性吸毒者的口腔中分离得到，其致病性可能低于从患有口咽念珠菌病的HIV阳性患者或患有侵袭性念珠菌病的HIV阴性患者中分离出的另一组的8株菌株。
