Yule T D, Roth M B, Dreier K, Johnson A F, Palmer-Densmore M, Simmons K, Fanton R
Central Research Division, Pfizer Inc., Groton, CT 06340, USA.
Vaccine. 1997 Apr-May;15(6-7):720-9. doi: 10.1016/s0264-410x(96)00232-0.
Inflammatory changes following infection are central to the clinical manifestation of disease. However, information regarding such changes in animal disease is limited. In canine parvovirus infected puppies we measured the levels of acute phase proteins and changes in leukocyte phenotypes and cell trafficking by flow cytometry. These parameters correlated with conventional assessment of clinical disease in a vaccine efficacy study. Seropositive (CPV-2) 6-week-old puppies given three doses of a CPV-2 containing vaccine developed significant antibody titers and remained healthy after experimental infection with CPV-2b. Unvaccinated controls developed clinical signs and shed virus. Importantly, acute phase proteins became elevated, and lymphopenia, neutropenia and modulation of neutrophil-CD4 were detected in controls but not in vaccinates.
感染后的炎症变化是疾病临床表现的核心。然而，关于动物疾病中此类变化的信息有限。在感染犬细小病毒的幼犬中，我们通过流式细胞术测量了急性期蛋白水平以及白细胞表型和细胞迁移的变化。在一项疫苗效力研究中，这些参数与临床疾病的传统评估相关。给予三剂含犬细小病毒2型疫苗的血清阳性（CPV-2）6周龄幼犬产生了显著的抗体滴度，并且在经CPV-2b实验性感染后保持健康。未接种疫苗的对照组出现临床症状并排出病毒。重要的是，对照组中急性期蛋白升高，并且检测到淋巴细胞减少、中性粒细胞减少以及中性粒细胞-CD4的调节，而接种疫苗的幼犬中未出现这些情况。
