Jackson A C, Rossiter J P
Department of Medicine, Queen's University, Kingston, Ontario, Canada.
J Virol. 1997 Jul;71(7):5603-7. doi: 10.1128/JVI.71.7.5603-5607.1997.
Cultured rat prostatic adenocarcinoma (AT3) cells infected with the challenge virus standard (CVS) strain of fixed rabies virus showed characteristic morphologic features of apoptosis, evidence of oligonucleosomal DNA fragmentation, and expression of the Bax protein. CVS-infected Bcl-2-transfected AT3 cells did not demonstrate these features. Adult ICR mice inoculated intracerebrally with CVS showed morphologic changes of apoptosis, DNA fragmentation, and increased Bax expression in neurons, with changes most marked in the hippocampus and cerebral cortex. Ultrastructurally, some neurons demonstrated morphologic features more typical of necrosis. These studies provide evidence that apoptosis plays an important role in the pathogenesis of rabies virus infection.
用狂犬病固定毒标准攻击病毒株（CVS）感染的培养大鼠前列腺腺癌（AT3）细胞呈现出凋亡的特征性形态学特征、寡核小体DNA片段化的证据以及Bax蛋白的表达。感染CVS的Bcl-2转染AT3细胞未表现出这些特征。经脑内接种CVS的成年ICR小鼠在神经元中呈现出凋亡的形态学变化、DNA片段化以及Bax表达增加，其中海马体和大脑皮层的变化最为明显。在超微结构上，一些神经元表现出更典型的坏死形态学特征。这些研究提供了证据表明凋亡在狂犬病病毒感染的发病机制中起重要作用。
