Shin K Y, Kong G, Kim W S, Lee T Y, Woo Y N, Lee J D
Department of Urology, College of Medicine, Hanyang University, Seoul, Korea.
Br J Cancer. 1997;75(12):1788-92. doi: 10.1038/bjc.1997.305.
Cyclin D1 is a cell cycle regulator essential for G1 phase progression and is frequently overexpressed in several human tumour types as a consequence of gene amplification or chromosomal rearrangements. We analysed the expression of cyclin D1 in 75 patients with transitional cell carcinoma (TCC) to investigate the possible relationship between its expression and clinical outcome as well as histopathological findings using the immunohistochemical method. We observed strong staining (++, > 50% positive cells) for cyclin D1 in 19 cases (25.3%) and weak staining (+, 5-50% positive cells) in 19 cases (25.3%). Overexpression of cyclin D1 was not associated with tumour invasion. No significant association was found between overexpression of cyclin D1 and tumour grade (P > 0.05). We assessed the differences of disease-free interval in superficial tumours and actuarial survival probability in invasive tumours according to the status of cyclin D1 expression. Tumours with (++) staining for cyclin D1 recurred much more rapidly than (-) and/or (+) staining tumours (P < 0.01 for - vs ++; P < 0.05 for + vs ++). However, overexpression of cyclin D1 was not associated with a shortened overall survival of patients with invasive tumours (P < 0.1). These results suggest that genetic alteration of cyclin D1 appears to be an early event in the tumorigenesis of bladder TCC and is associated with early recurrence in superficial tumours.
细胞周期蛋白D1是一种对G1期进程至关重要的细胞周期调节因子，由于基因扩增或染色体重排，它在几种人类肿瘤类型中经常过度表达。我们采用免疫组织化学方法分析了75例移行细胞癌（TCC）患者中细胞周期蛋白D1的表达情况，以研究其表达与临床结果以及组织病理学发现之间的可能关系。我们观察到19例（25.3%）患者的细胞周期蛋白D1呈强染色（++，阳性细胞>50%），19例（25.3%）呈弱染色（+，阳性细胞5%-50%）。细胞周期蛋白D1的过度表达与肿瘤侵袭无关。细胞周期蛋白D1的过度表达与肿瘤分级之间未发现显著关联（P>0.05）。我们根据细胞周期蛋白D1的表达状态评估了浅表肿瘤无病间期的差异以及浸润性肿瘤的精算生存概率。细胞周期蛋白D1染色为（++）的肿瘤比（-）和/或（+）染色的肿瘤复发快得多（-与++相比，P<0.01；+与++相比，P<0.05）。然而，细胞周期蛋白D1的过度表达与浸润性肿瘤患者的总生存期缩短无关（P<0.1）。这些结果表明，细胞周期蛋白D1的基因改变似乎是膀胱TCC肿瘤发生过程中的早期事件，并且与浅表肿瘤的早期复发有关。
