白色念珠菌延伸因子3基因启动子序列中的微卫星多态性作为一种分型系统的基础。
Microsatellite polymorphism in the promoter sequence of the elongation factor 3 gene of Candida albicans as the basis for a typing system.
作者信息
Bretagne S, Costa J M, Besmond C, Carsique R, Calderone R
机构信息
Laboratoire de Parasitologie-Mycologie, Hôpital Henri Mondor-Assistance Publique, Créteil, France.
出版信息
J Clin Microbiol. 1997 Jul;35(7):1777-80. doi: 10.1128/jcm.35.7.1777-1780.1997.
Abstract
The polymorphism of a TTC/TTTC microsatellite in the promoter sequence of the elongation factor 3 gene of Candida albicans was investigated by PCR. One primer was fluorescein labeled, and PCR signals were read with an automatic sequencer. Twenty-nine reference strains and 31 independent clinical isolates were studied. Eleven different alleles were identified, giving 16 different profiles among the 60 strains tested, with a discriminatory power of 0.88. This marker is stable upon subculture, and reproducibility was achieved by automated procedures. When several microsatellite markers are available, many isolates can be rapidly and reproducibly tested for epidemiological questions, such as the prevalence of a given strain in a hospital setting and transmission between patients.
摘要
采用聚合酶链反应（PCR）研究了白色念珠菌延伸因子3基因启动子序列中TTC/TTTC微卫星的多态性。其中一条引物用荧光素标记，PCR信号通过自动测序仪读取。研究了29株参考菌株和31株独立的临床分离株。鉴定出11种不同的等位基因，在60株测试菌株中产生了16种不同的图谱，鉴别力为0.88。该标记在传代培养时稳定，通过自动化程序可实现重复性。当有多个微卫星标记可用时，许多分离株可快速且可重复地用于流行病学问题的检测，例如医院环境中特定菌株的流行情况以及患者之间的传播。
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