加纳人类免疫缺陷病毒1型的系统发育分析。

Phylogenetic analysis of human immunodeficiency virus 1 in Ghana.

作者信息

Takehisa J, Osei-Kwasi M, Ayisi N K, Hishida O, Miura T, Igarashi T, Brandful J, Ampofo W, Netty V B, Mensah M, Yamashita M, Ido E, Hayami M

机构信息

Laboratory of Pathogenic Virus, Kyoto University, Japan.

出版信息

Acta Virol. 1997 Feb;41(1):51-4.

PMID:9199716

Abstract

Eleven human immunodeficiency virus 1 (HIV-1) isolates from Ghanaian acquired immunodeficiency syndrome (AIDS) or AIDS-related complex (ARC) patients obtained by our serosurvey in 1986-1994 were genomically analyzed and phylogenetically compared with other known strains. A phylogenetic tree constructed by analyzing the env region indicated that heterogeneous HIV-1 strains were circulating in Ghana and the majority of them (9 of 11 isolates) belonged to clade (subtype) A which is now furiously epidemic in Africa. Another isolate (1 of 11) belonged to clade D, and the remaining one (1 of 11) belonged to "clade G". This "clade G" virus grouped by the env analysis belonged to clade A by its pol sequence, suggesting an A/G intersubtype recombinant. The characteristic sequences in the V3 tip which have not yet been reported were observed in these Ghanaian isolates, which should be taken into account for future vaccine programs.

摘要

对1986年至1994年我们通过血清学调查从加纳获得性免疫缺陷综合征（AIDS）或艾滋病相关综合征（ARC）患者中分离出的11株人类免疫缺陷病毒1型（HIV-1）进行了基因组分析，并与其他已知毒株进行了系统发育比较。通过分析env区域构建的系统发育树表明，不同的HIV-1毒株在加纳流行，其中大多数（11株分离株中的9株）属于A组（亚型），该组目前在非洲正疯狂流行。另一株分离株（11株中的1株）属于D组，其余一株（11株中的1株）属于“G组”。通过env分析分组的这种“G组”病毒，其pol序列属于A组，提示为A/G亚型间重组体。在这些加纳分离株中观察到了尚未报道的V3尖端特征序列，这在未来的疫苗计划中应予以考虑。