Crespo J F, Pascual C, Helm R, Sanchez-Pastor S, Ojeda I, Romualdo L, Martin-Esteban M, Ojeda J A
Allergy Division, La Paz Children's Hospital, Madrid, Spain.
Allergy. 1995 Nov;50(11):918-24. doi: 10.1111/j.1398-9995.1995.tb02499.x.
IgE-antibody reactivity to boiled Atlantic shrimp (Pandalus borealis) and German cockroach (Blattella germanica) of sera from 89 patients, sensitive to one or the other, was investigated with an enzymatic immunoassay for specific IgE detection (CAP-FEIA System, Pharmacia, Sweden). IgE serum levels to both antishrimp and anticockroach allergens were found to be positive in 76 of the 89 (85.4%) tested sera. A positive anticockroach IgE was very rare in the absence of detectable antishrimp IgE (five of 89 sera). Linear regression analysis on antishrimp and anti-German cockroach IgE levels-log plot revealed a correlation coefficient (r) of 0.73. Inhibition experiments showed that boiled Atlantic shrimp extract inhibited CAP with German cockroach, and vice versa. Immunoblotting showed the strongest IgE binding for both allergenic extracts between 30 and 43 kDa. By blot inhibition, the binding capacity of German cockroach was totally abolished by Atlantic shrimp extract, while German cockroach extract only partially IgE binding to Atlantic shrimp. Cross-reactivity exists between shrimp, an important food allergen, and German cockroach, which has an increasing role in allergic asthma. It could be important to determine the clinical significance of cross-allergy to both allergens, in which exposures occur in different ways.
采用酶免疫分析法（瑞典法玛西亚公司的CAP - FEIA系统）对89例对大西洋虾或德国小蠊敏感的患者血清中针对煮熟的大西洋虾（北方褐虾）和德国小蠊的IgE抗体反应性进行了研究。在89份检测血清中，有76份（85.4%）的抗虾和抗蟑螂过敏原的IgE血清水平呈阳性。在未检测到抗虾IgE的情况下，抗蟑螂IgE呈阳性的情况非常罕见（89份血清中有5份）。对抗虾和抗德国小蠊IgE水平对数图进行线性回归分析，得出相关系数（r）为0.73。抑制实验表明，煮熟的大西洋虾提取物可抑制针对德国小蠊的CAP，反之亦然。免疫印迹显示，两种变应原提取物在30至43 kDa之间的IgE结合最强。通过印迹抑制法，大西洋虾提取物可完全消除德国小蠊的结合能力，而德国小蠊提取物只能部分抑制IgE与大西洋虾的结合。重要的食物过敏原虾和在过敏性哮喘中作用日益增加的德国小蠊之间存在交叉反应性。确定对这两种变应原交叉过敏的临床意义可能很重要，因为对它们的接触是以不同方式发生的。
