Grilló M J, Barberán M, Blasco J M
Department of Animal Health, SIA/DGA, Zaragoza, Spain.
Vet Rec. 1997 Jun 7;140(23):602-5. doi: 10.1136/vr.140.23.602.
Forty-one pregnant sheep showing positive immune responses to Brucella melitensis in serological or allergic tests were selected from naturally infected flocks and kept in an isolated pen for lambing. The resulting 62 lambs were maintained in the same pen with their dams during lactation. When the lambs were weaned, the dams were slaughtered for bacteriological study and the lambs were reared in a clean pen. Fourteen ewes excreted B melitensis during lactation and 17 were found to be infected postmortem, B melitensis was not isolated from seven lambs (three born to infected dams) which died after birth or from eight seronegative lambs (four born to infected dams) which were slaughtered between two and seven months after weaning. However, one permanently seropositive lamb born to a culture-negative dam was found to be infected when necropsied five months after weaning. The remaining 46 lambs were reared until adulthood and slaughtered at intervals for bacteriological study. Four ewe lambs (two born to culture-negative dams) were found to be infected postmortem, but were negative in immunological tests for B melitensis.
从自然感染的羊群中挑选出41只在血清学或变态反应试验中对布鲁氏菌呈阳性免疫反应的怀孕绵羊，将它们关在一个隔离的围栏中待产。所产的62只羔羊在哺乳期与母羊一起饲养在同一个围栏中。羔羊断奶后，将母羊宰杀进行细菌学研究，羔羊则在一个干净的围栏中饲养。14只母羊在哺乳期排出布鲁氏菌，17只在死后被发现感染，7只出生后死亡的羔羊（3只出生于感染母羊）以及8只断奶后2至7个月间被宰杀的血清学阴性羔羊（4只出生于感染母羊）未分离出布鲁氏菌。然而，1只出生于培养阴性母羊的永久血清学阳性羔羊在断奶后5个月进行尸检时被发现感染。其余46只羔羊饲养至成年，定期宰杀进行细菌学研究。4只母羊羔（2只出生于培养阴性母羊）在死后被发现感染，但在布鲁氏菌免疫试验中呈阴性。
