Hermonat P L, Han L, Wendel P J, Quirk J G, Stern S, Lowery C L, Rechtin T M
Department of Obstetrics and Gynecology, University of Arkansas for Medical Sciences, Little Rock 72205, USA.
Virus Genes. 1997;14(1):13-7. doi: 10.1023/a:1007975005433.
In this study the possible role of human papillomaviruses (HPV) in spontaneous abortions is addressed by assaying for HPV DNA in first trimester spontaneous and electively aborted products of conception materials enriched for chorionic villi. The presence of HPVs was measured by polymerase chain reaction (PCR) amplification and DNA dot blot hybridization using an internal probe. The "broad spectrum" HPV primers were directed to amplify E6/E7 junction sequences, while the probe was of an HPV-16 sequence with significant homology to HPV-6/11. The quantity and quality of isolated DNA was also analyzed and compared by observing the PCR amplification of a cellular sequence from the human beta-globin gene. Fifteen of the 25 spontaneous samples (60%) were found to be positive for HPV E6/E7 sequences. In comparison, only 3 of the 15 elective samples (20%) were positive. This is the first study of HPV in fetal materials to incorporate material from elective abortions as a control group. Although confounding contamination from the cervix and vagina can't be ruled out, these data are significant and strongly suggest that HPVs are elevated in spontaneously aborted products of conception. Furthermore, these results suggest the possibility that HPVs may be etiologic agents of at least some spontaneous abortions.
在本研究中，通过检测孕早期自然流产和人工流产的富含绒毛膜绒毛的妊娠产物中的人乳头瘤病毒（HPV）DNA，探讨了HPV在自然流产中的可能作用。使用内部探针，通过聚合酶链反应（PCR）扩增和DNA斑点印迹杂交来检测HPV的存在。“广谱”HPV引物用于扩增E6/E7连接序列，而探针是与HPV-6/11具有显著同源性的HPV-16序列。还通过观察人β-珠蛋白基因细胞序列的PCR扩增，对分离的DNA的数量和质量进行了分析和比较。25个自然流产样本中有15个（60%）HPV E6/E7序列呈阳性。相比之下，15个人工流产样本中只有3个（20%）呈阳性。这是首次在胎儿材料中研究HPV，并将人工流产材料作为对照组。尽管不能排除来自宫颈和阴道的混杂污染，但这些数据具有重要意义，并强烈表明自然流产的妊娠产物中HPV水平升高。此外，这些结果提示HPV可能是至少某些自然流产的病因。
