Cerná M, Pastorková A, Myers S R, Rössner P, Binková B
National Institute of Public Health, Division of Environmental Health, Prague, Czech Republic.
Mutat Res. 1997 Jun 13;391(1-2):99-110. doi: 10.1016/s1383-5718(97)00058-2.
Urinary bacterial mutagenicity was used as a biomarker of exposure to ambient air pollution in a group of women working outdoors in the city of Teplice (TP; Northern Bohemia) with higher levels of air pollution than a similar group of women in the city of Prachatice (PT; Southern Bohemia). The Salmonella typhimurium plate incorporation assay with the TA98 and YG1041 strains and microsuspension assay with the YG1041 strain were used for testing the urinary mutagenicity. PAH and their metabolites were analyzed by HPLC and GC/MS methods. The significantly higher values of most PAHs/metabolites detected in a TP group confirmed the differences of PAH exposures between both groups. In the plate incorporation assay, the TA98 strain was not able to detect the increase in urinary mutagenicity, but, for the YG1041 strain, the urinary mutagenicity was clearly determined with a significant difference in number of YG1041 + S9 revertants between the TP and PT groups. The microsuspension assay increased the mean response by about 10-fold over the standard plate test; however, no statistical difference between TP and PT groups was found due to high interindividual variability and small sample size. Comparing the urinary PAH/metabolites to urinary mutagenicity, significant correlations were observed between the plate incorporation mutagenicity results with the YG1041 revertants in the presence of metabolic activation and several of the urinary PAH/metabolites. On the contrary, in the microsuspension assay, several urinary PAH/metabolites correlated significantly with the YG1041 revertants only in the absence of metabolic activation. This may indicate the influence of different treatment conditions of assays on the urinary mutagenicity results. The results suggest the insufficient sensitivity of the TA98 tester strain to determinate low urinary level of mutagens. On the contrary, the use of the YG1041 tester strain increases the probability of detecting an effect of environmental exposure and seems to be applicable to biological monitoring. To definitely replace the standard plate incorporation assay with the microsuspension method is not possible without further comparative studies.
在捷克北部城市特普利采（TP），一组从事户外工作的女性暴露于空气污染水平较高的环境中，而在捷克南部城市普拉哈蒂采（PT），一组类似的女性暴露于空气污染水平较低的环境中。尿细菌致突变性被用作暴露于环境空气污染的生物标志物。采用鼠伤寒沙门氏菌平板掺入试验（TA98和YG1041菌株）以及YG1041菌株的微悬浮试验来检测尿致突变性。通过高效液相色谱法（HPLC）和气相色谱/质谱法（GC/MS）分析多环芳烃（PAH）及其代谢产物。在TP组中检测到的大多数PAH/代谢产物的值显著更高，这证实了两组之间PAH暴露的差异。在平板掺入试验中，TA98菌株无法检测到尿致突变性的增加，但对于YG1041菌株，尿致突变性可以明确测定，TP组和PT组之间YG1041 + S9回复突变体的数量存在显著差异。微悬浮试验使平均反应比标准平板试验增加了约10倍；然而，由于个体间变异性高和样本量小，未发现TP组和PT组之间存在统计学差异。将尿PAH/代谢产物与尿致突变性进行比较，在代谢激活存在的情况下，平板掺入致突变性结果与YG1041回复突变体以及几种尿PAH/代谢产物之间观察到显著相关性。相反，在微悬浮试验中，几种尿PAH/代谢产物仅在没有代谢激活的情况下与YG1041回复突变体显著相关。这可能表明试验的不同处理条件对尿致突变性结果的影响。结果表明，TA98测试菌株对测定低水平尿致突变剂的敏感性不足。相反，使用YG1041测试菌株增加了检测环境暴露影响的可能性，似乎适用于生物监测。在没有进一步比较研究的情况下，不可能用微悬浮方法完全取代标准平板掺入试验。
