采用抗体捕获放射免疫分析法检测细小病毒B19特异性IgM。

Detection of parvovirus B19-specific IgM by antibody capture radioimmunoassay.

作者信息

Cohen B J

机构信息

Enteric and Respiratory Virus Laboratory, Central Public Health Laboratory, London, UK.

出版信息

J Virol Methods. 1997 Jun;66(1):1-4. doi: 10.1016/s0166-0934(97)00048-7.

DOI:10.1016/s0166-0934(97)00048-7

PMID:9220384

Abstract

A solid phase IgM-capture radioimmunoassay (MACRIA) for the detection of parvovirus B19-IgM is described (Cohen et al., 1983, J. Hyg. Camb. 91, 113-130). IgM from a dilution of patients serum is 'captured' onto a solid phase coated by anti-human IgM. To determine whether any of the IgM is specific for parvovirus B19, B19 antigen is added followed by a detector system. In the MACRIA described here the detector system comprises a mouse monoclonal antibody to parvovirus B19 and a 125I-labelled anti-mouse antibody. A calibration curve derived from a standard B19 IgM serum is used to quantify B19 IgM using a single dilution of test sera. The purpose of the protocol is the diagnosis of recent acute infection with parvovirus B19.

摘要

本文描述了一种用于检测细小病毒B19-IgM的固相IgM捕获放射免疫分析方法（MACRIA）（科恩等人，1983年，《剑桥卫生杂志》91卷，113 - 130页）。将患者血清稀释液中的IgM“捕获”到包被有抗人IgM的固相上。为了确定IgM中是否有任何针对细小病毒B19的特异性抗体，加入B19抗原，随后加入检测系统。在此描述的MACRIA中，检测系统包括一种针对细小病毒B19的小鼠单克隆抗体和一种125I标记的抗小鼠抗体。使用来自标准B19 IgM血清的校准曲线，通过对测试血清进行单次稀释来定量B19 IgM。该方案的目的是诊断近期细小病毒B19的急性感染。