Nualláin E M, Davis W C, Costello E, Pollock J M, Monaghan M L
Department of Large Animal Clinical Studies, Faculty of Veterinary Medicine, University College Dublin, Ballsbridge, Ireland.
Vet Immunol Immunopathol. 1997 May;56(1-2):65-76. doi: 10.1016/s0165-2427(96)05734-0.
After activation of T-lymphocytes with antigen there is an increase in the expression of interleukin-2 receptor-alpha (IL-2R-alpha) followed by the release of a soluble form of the molecule (sIL-2R-alpha) from the membrane of the stimulated cells. The present study investigates the novel use of the release of sIL-2R-alpha from activated T-lymphocytes as a marker of cell-mediated immunity (CMI) in cattle infected with Mycobacterium bovis. An enzyme immunoassay was used to detect sIL-2R-alpha produced following incubation of bovine peripheral blood mononuclear cells with mycobacterial antigens. Using this assay, 63/67 cattle naturally infected with M. bovis were identified whereas only 1/51 uninfected animals were considered to give a positive result. This assay is more convenient to use than lymphocyte proliferation assays which involve the use of radionucleosides. It should prove useful for monitoring the immunological activation of bovine T-lymphocytes in a variety of situations including the development of CMI responses in cattle to novel mycobacterial antigens or potential vaccines.
用抗原激活T淋巴细胞后，白细胞介素-2受体α（IL-2R-α）的表达增加，随后受刺激细胞的膜释放出该分子的可溶性形式（sIL-2R-α）。本研究调查了活化的T淋巴细胞释放sIL-2R-α作为牛分枝杆菌感染牛的细胞介导免疫（CMI）标志物的新用途。采用酶免疫分析法检测牛外周血单个核细胞与分枝杆菌抗原孵育后产生的sIL-2R-α。使用该检测方法，63/67头自然感染牛分枝杆菌的牛被鉴定出来，而51头未感染动物中只有1头被认为检测结果呈阳性。该检测方法比涉及使用放射性核素的淋巴细胞增殖检测方法更便于使用。它应被证明在监测牛T淋巴细胞在各种情况下的免疫激活方面是有用的，包括牛对新型分枝杆菌抗原或潜在疫苗的CMI反应的发展。
