芦笋中的甾体皂苷及其细胞毒性活性。

Steroidal saponins from Asparagus officinalis and their cytotoxic activity.

作者信息

Shao Y, Poobrasert O, Kennelly E J, Chin C K, Ho C T, Huang M T, Garrison S A, Cordell G A

机构信息

Department of Plant Science, Cook College, Rutgers, State University of New Jersey, New Brunswick 08903, USA.

出版信息

Planta Med. 1997 Jun;63(3):258-62. doi: 10.1055/s-2006-957667.

DOI:10.1055/s-2006-957667

PMID:9225609

Abstract

Two oligofurostanosides were isolated from the seeds of Asparagus officinalis L and their structures characterized as 3-O-[alpha-L-rhamnopyranosyl-(1-->2)-(alpha-L-rhamnopyranosyl- (1-->4))-beta-D-glucopyranosyl]-26-O-[beta-D-glucopyranosyl]-(25R) -22 alpha-methoxyfurost-5-ene-3 beta,26-diol(methyl protodioscin) and its corresponding 22 alpha-hydroxy analogue (protodioscin). The structural identification was performed using detailed analysis of 1H- and 13C-NMR spectra including two-dimensional NMR spectroscopy (COSY, HMQC, NOESY and HMBC), and chemical conversions. These two compounds have been shown to inhibit the growth of human leukemia HL-60 cells in culture and macromolecular synthesis in a dose-dependent manner. The inhibitory effect on DNA synthesis was found to be irreversible.

摘要

从芦笋种子中分离出两种寡呋喃甾苷，其结构被鉴定为3 - O - [α - L - 鼠李吡喃糖基 - (1→2) - (α - L - 鼠李吡喃糖基 - (1→4)) - β - D - 葡萄糖吡喃糖基] - 26 - O - [β - D - 葡萄糖吡喃糖基] - (25R) - 22α - 甲氧基呋甾 - 5 - 烯 - 3β,26 - 二醇（甲基原薯蓣皂苷）及其相应的22α - 羟基类似物（原薯蓣皂苷）。通过对1H - 和13C - NMR光谱进行详细分析，包括二维NMR光谱（COSY、HMQC、NOESY和HMBC）以及化学转化来进行结构鉴定。已证明这两种化合物在培养中能以剂量依赖性方式抑制人白血病HL - 60细胞的生长和大分子合成。发现其对DNA合成的抑制作用是不可逆的。