Zhang Ruo-Song, Liu Yang-Yang, Zhu Pei-Feng, Jin Qiong, Dai Zhi, Luo Xiao-Dong
State Key Laboratory of Phytochemistry and Plant Resources in West China and Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
Nat Prod Bioprospect. 2021 Dec;11(6):651-658. doi: 10.1007/s13659-021-00321-0. Epub 2021 Nov 5.
Phytochemical investigation on the roots of Asparagus cochinchinensis led to the isolation of one new furostanol saponin, named 26-O-β-D-glucopyranosyl-22α-hydroxyl-(25R)-Δ-furost-3β,26-diol-3-O-α-L-rhamnopyranosyl-(1 → 2)-[β-D-glucopyranosyl-(1 → 4)-α-L-rhamnopyranosyl-(1 → 4)]-β-D-glucopyranoside (1), along with three known congeners (2‒4). The structure of new saponin was elucidated via comprehensive inspection of its HRMS and NMR spectral data as well as chemical technology, whereas those of known ones were identified by comparison of their NMR and MS spectral data with those reported in literatures. All isolated saponins were evaluated for their cytotoxic effects on two human liver (MHCC97H) and lung adenocarcinoma (H1299) cancer cells in vitro. Among them, both 1 and 2 showed significant cytotoxicity against above mentioned cell lines. Further studies revealed that these two saponins could significantly inhibit their proliferation of MHCC97H and H1299 cells.
对天门冬根进行植物化学研究，从中分离出一种新的呋甾烷醇皂苷，命名为26 - O - β - D - 吡喃葡萄糖基 - 22α - 羟基 - (25R) - Δ - 呋甾 - 3β,26 - 二醇 - 3 - O - α - L - 吡喃鼠李糖基 - (1→2) - [β - D - 吡喃葡萄糖基 - (1→4) - α - L - 吡喃鼠李糖基 - (1→4)] - β - D - 吡喃葡萄糖苷（1），以及三种已知的同系物（2 - 4）。通过对其高分辨质谱和核磁共振光谱数据以及化学方法的综合分析阐明了新皂苷的结构，而通过将已知同系物的核磁共振和质谱数据与文献报道的数据进行比较来鉴定其结构。对所有分离得到的皂苷进行了体外对两种人肝癌（MHCC97H）和肺腺癌（H1299）癌细胞的细胞毒性作用评估。其中，1和2对上述细胞系均表现出显著的细胞毒性。进一步研究表明，这两种皂苷可显著抑制MHCC97H和H1299细胞的增殖。
