Bradley W P, Blasco A P, Weiss J F, Alexander J C, Silverman N A, Chretien P B
Cancer. 1977 Nov;40(5):2264-72. doi: 10.1002/1097-0142(197711)40:5<2264::aid-cncr2820400537>3.0.co;2-3.
Levels of glycoprotein-associated carbohydrates (neutral hexoses, hexosamine, sialic acid and fucose) were determined in the serum of patients with either local, regional or metastatic cancer, patients clinically cured of cancer, and controls (smokers and nonsmokers). Total protein-bound carbohydrates were compared with levels of 17 normal serum glycoproteins, carcinoembryonic antigen (CEA), and with lymphocyte reactivity to phytohemagglutin (PHA). Tumor burden was directly related to protein-bound carbohydrate levels in patient groups. Levels of bound carbohydrates reflect the sum of all the changes in serum glycoproteins, but primarily changes in the acute-phase proteins (alpha 1-acid glycoprotein, alpha 1-antitrypsin, haptoglobin, ceruloplasmin) found in the alpha-globulin fraction of serum. Increases in protein-bound carbohydrates in tumor-bearers were not related to increases in CEA. Increased levels of the acute-phase proteins occurred in individuals with depressed in vitro lymphocyte reactivity to PHA. A significant positive correlation was found between lymphocyte reactivity and level of alpha 2HS-glycoprotein. The results suggest that serum protein-bound carbohydrates or glycoproteins may be of adjunctive value is assessing tumor burden and immune reactivity in cancer patients.
测定了患有局部、区域或转移性癌症的患者、临床治愈的癌症患者以及对照组（吸烟者和非吸烟者）血清中糖蛋白相关碳水化合物（中性己糖、己糖胺、唾液酸和岩藻糖）的水平。将总蛋白结合碳水化合物与17种正常血清糖蛋白、癌胚抗原（CEA）的水平以及淋巴细胞对植物血凝素（PHA）的反应性进行了比较。肿瘤负荷与患者组中蛋白结合碳水化合物水平直接相关。结合碳水化合物的水平反映了血清糖蛋白所有变化的总和，但主要反映了血清α球蛋白部分中急性期蛋白（α1-酸性糖蛋白、α1-抗胰蛋白酶、触珠蛋白、铜蓝蛋白）的变化。肿瘤患者中蛋白结合碳水化合物的增加与CEA的增加无关。急性期蛋白水平的升高发生在体外淋巴细胞对PHA反应性降低的个体中。发现淋巴细胞反应性与α2HS-糖蛋白水平之间存在显著正相关。结果表明，血清蛋白结合碳水化合物或糖蛋白在评估癌症患者的肿瘤负荷和免疫反应性方面可能具有辅助价值。
