Kamekura M, Dyall-Smith M L, Upasani V, Ventosa A, Kates M
Noda Institute for Scientific Research, Japan.
Int J Syst Bacteriol. 1997 Jul;47(3):853-7. doi: 10.1099/00207713-47-3-853.
The 16S rRNA genes of three species of the genus Natronobacterium (Natronobacterium gregoryi, Natronobacterium pharaonis, and Natronobacterium vacuolatum) were sequenced and compared to that of the previously sequenced species Natronobacterium magadii. The sequences revealed that Natronobacterium pharaonis was phylogenetically distinct from the other members of the genus and also from other recognized genera of the family Halobacteriaceae. However, Natronobacterium vacuolatum and Natronobacterium magadii were found to be most closely related to the genera Halorubrum and Natrialba, respectively. An unidentified haloalkaliphile, strain SSL1, was also closely related to Natronobacterium magadii and Natrialba asiatica. On the basis of phylogenetic tree reconstructions, signature bases specific for individual genera, and sequences of spacer regions between 16 and 23S rRNA genes, we propose the following changes: Natronobacterium pharaonis to be transferred to Natronomonas gen. nov. as Natronomonas pharaonis gen. nov., comb. nov.; Natronobacterium vacuolatum to be transferred to the genus Halorubrum as Halorubrum vacuolatum comb. nov.; and Natronobacterium magadii to be transferred to the genus Natrialba as Natrialba magadii.
对嗜盐碱芽孢杆菌属（嗜盐碱芽孢杆菌、法老嗜盐碱芽孢杆菌和泡状嗜盐碱芽孢杆菌）三个物种的16S rRNA基因进行了测序，并与之前已测序的嗜盐碱芽孢杆菌物种进行了比较。序列显示，法老嗜盐碱芽孢杆菌在系统发育上与该属的其他成员以及盐杆菌科其他公认的属不同。然而，发现泡状嗜盐碱芽孢杆菌和嗜盐碱芽孢杆菌分别与嗜盐红菌属和嗜盐碱球菌属关系最为密切。一种未鉴定的嗜盐碱嗜盐菌SSL1菌株也与嗜盐碱芽孢杆菌和亚洲嗜盐碱球菌密切相关。基于系统发育树重建、各属特异性的特征碱基以及16S和23S rRNA基因之间间隔区的序列，我们提出以下分类变动：将法老嗜盐碱芽孢杆菌转移至新属嗜盐碱单胞菌属，命名为法老嗜盐碱单胞菌，新组合；将泡状嗜盐碱芽孢杆菌转移至嗜盐红菌属，命名为泡状嗜盐红菌，新组合；将嗜盐碱芽孢杆菌转移至嗜盐碱球菌属，命名为亚洲嗜盐碱球菌。
