通过16S rRNA测序鉴定在日本分离出的立克次氏体为贝氏柯克斯体。
Identification of rickettsiae isolated in Japan as Coxiella burnetii by 16S rRNA sequencing.
作者信息
Masuzawa T, Sawaki K, Nagaoka H, Akiyama M, Hirai K, Yanagihara Y
机构信息
Department of Microbiology, School of Pharmaceutical Sciences, University of Shizuoka, Japan.
出版信息
Int J Syst Bacteriol. 1997 Jul;47(3):883-4. doi: 10.1099/00207713-47-3-883.
The 16S rRNA genes of Japanese Coxiella isolates obtained from various sources and geographical areas were directly sequenced by dideoxynucleotide chain termination methods in which Taq DNA polymerase was used. The levels of sequence similarity among Japanese, European, and American isolates were more than 99%, and the Japanese isolates were identified as Coxiella burnetii, C. burnetii strains isolated worldwide, including Japan, were found to be very similar.
采用双脱氧核苷酸链终止法并使用Taq DNA聚合酶，直接对从日本不同来源和地理区域获得的柯克斯体分离株的16S rRNA基因进行测序。日本、欧洲和美国分离株之间的序列相似性水平超过99%，并且日本分离株被鉴定为贝氏柯克斯体，发现包括日本在内全世界分离出的贝氏柯克斯体菌株非常相似。
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