利用空斑试验培养法在24小时内对呼吸道病毒感染进行实验室诊断。
Laboratory diagnosis of respiratory virus infections in 24 hours by utilizing shell vial cultures.
作者信息
Engler H D, Preuss J
机构信息
Clinical Pathology Department, Warren Grant Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892-1508, USA.
出版信息
J Clin Microbiol. 1997 Aug;35(8):2165-7. doi: 10.1128/jcm.35.8.2165-2167.1997.
Abstract
Immunofluorescence staining of centrifugation-enhanced shell vial (SV) cultures for respiratory viruses (RV) after 24 h of incubation, rather than the more commonly prescribed times of 48 h and 5 days, allowed for the detection of 77% of the RV-positive specimens that would ordinarily not have been detected as positive until 48 h. Staining SVs at 24 h also permitted earlier detection of viruses that were missed by rapid antigen detection methods.
摘要
对呼吸道病毒（RV）进行离心增强空斑培养（SV），培养24小时后进行免疫荧光染色，而非更常用的48小时和5天的培养时间，能够检测出77%的RV阳性标本，这些标本通常在48小时前不会被检测为阳性。在24小时对SV进行染色，还能更早地检测出快速抗原检测方法遗漏的病毒。
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Rapid diagnosis of respiratory viral infections by using a shell vial assay and monoclonal antibody pool.使用空斑试验和单克隆抗体库快速诊断呼吸道病毒感染
J Clin Microbiol. 1992 Jun;30(6):1505-8. doi: 10.1128/jcm.30.6.1505-1508.1992.
8
Rapid detection of respiratory viruses by shell vial culture and direct staining by using pooled and individual monoclonal antibodies.通过空斑试验培养并使用混合及单克隆抗体进行直接染色来快速检测呼吸道病毒。
J Clin Microbiol. 1992 Mar;30(3):540-4. doi: 10.1128/jcm.30.3.540-544.1992.
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