以3500×g离心塑料培养瓶对临床标本中病毒检测的影响。
Effect of centrifuging shell vials at 3,500 x g on detection of viruses in clinical specimens.
作者信息
Engler H D, Selepak S T
机构信息
Clinical Pathology Department, Warren Grant Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892-0001.
出版信息
J Clin Microbiol. 1994 Jun;32(6):1580-2. doi: 10.1128/jcm.32.6.1580-1582.1994.
Abstract
An increase in shell vial centrifugation force to 3,500 x g and a concomitant reduction in spin time to 15 min did not decrease the sensitivity of detecting viruses in clinical specimens compared with the accepted practice of using 700 x g for 40 min. No damage to the cell monolayer (ML) at the higher g force was observed. Toxicity to the ML is decreased with the shorter spin, probably because of reduced time of contact between the specimen and the ML.
摘要
与采用700×g离心40分钟的公认做法相比，将空斑离心力提高到3500×g并同时将离心时间缩短至15分钟，并未降低临床标本中病毒检测的灵敏度。在较高的重力下未观察到细胞单层（ML）受损。较短的离心时间降低了对细胞单层的毒性，这可能是因为标本与细胞单层的接触时间减少了。
相似文献
1
Effect of centrifuging shell vials at 3,500 x g on detection of viruses in clinical specimens.以3500×g离心塑料培养瓶对临床标本中病毒检测的影响。
J Clin Microbiol. 1994 Jun;32(6):1580-2. doi: 10.1128/jcm.32.6.1580-1582.1994.
2
Isolation of seven respiratory viruses in shell vials: a practical and highly sensitive method.在空斑试验瓶中分离七种呼吸道病毒：一种实用且高度灵敏的方法。
J Clin Microbiol. 1993 Feb;31(2):422-5. doi: 10.1128/jcm.31.2.422-425.1993.
3
Enhanced detection of respiratory viruses using the shell vial technique and monoclonal antibodies.使用空斑试验技术和单克隆抗体增强呼吸道病毒检测。
J Virol Methods. 1992 Sep;39(1-2):39-46. doi: 10.1016/0166-0934(92)90123-u.
4
Rapid detection of respiratory viruses using mixtures of monoclonal antibodies on shell vial cultures.在空斑小瓶培养物上使用单克隆抗体混合物快速检测呼吸道病毒。
J Med Virol. 1992 Oct;38(2):147-51. doi: 10.1002/jmv.1890380214.
5
Pooled nasopharyngeal and oropharyngeal samples for the identification of respiratory viruses in adults.用于鉴定成人呼吸道病毒的鼻咽和口咽合并样本。
Eur J Clin Microbiol Infect Dis. 2010 Jun;29(6):733-5. doi: 10.1007/s10096-010-0903-5. Epub 2010 Mar 12.
6
Specimen collection for virological tests.
Nurs Stand. 1991;5(37):28-31. doi: 10.7748/ns.5.37.28.s48.
7
Rapid detection of respiratory viruses by shell vial culture and direct staining by using pooled and individual monoclonal antibodies.通过空斑试验培养并使用混合及单克隆抗体进行直接染色来快速检测呼吸道病毒。
J Clin Microbiol. 1992 Mar;30(3):540-4. doi: 10.1128/jcm.30.3.540-544.1992.
8
eSwab flocked swabs unfit for viral culture.eSwab植绒拭子不适用于病毒培养。
J Clin Virol. 2012 Nov;55(3):282-3. doi: 10.1016/j.jcv.2012.07.006. Epub 2012 Aug 2.
9
Evaluation of number of shell vial cell cultures per clinical specimen for rapid diagnosis of cytomegalovirus infection.评估每份临床标本用于巨细胞病毒感染快速诊断的空斑小室细胞培养数量。
J Clin Microbiol. 1988 Feb;26(2):198-200. doi: 10.1128/jcm.26.2.198-200.1988.
10
Detection of enteroviruses from clinical specimens by spin amplification shell vial culture and monoclonal antibody assay.通过旋转扩增空斑培养和单克隆抗体检测法从临床标本中检测肠道病毒。
J Clin Microbiol. 1996 Jun;34(6):1465-7. doi: 10.1128/jcm.34.6.1465-1467.1996.
引用本文的文献
1
Enzyme linked oligonucleotide assay for the sensitive detection of SARS-CoV-2 variants.酶联寡核苷酸检测法用于灵敏检测 SARS-CoV-2 变体。
Front Cell Infect Microbiol. 2022 Sep 29;12:1017542. doi: 10.3389/fcimb.2022.1017542. eCollection 2022.
2
Validation of a multiplex reverse transcriptase PCR ELISA for the detection of 19 respiratory tract pathogens.一种用于检测 19 种呼吸道病原体的多重逆转录酶 PCR ELISA 的验证。
Infection. 2013 Feb;41(1):77-91. doi: 10.1007/s15010-012-0298-6. Epub 2012 Jul 31.
3
Shell Vial Culture assay for the rapid diagnosis of Japanese encephalitis, West Nile and Dengue-2 viral encephalitis.用于快速诊断日本脑炎、西尼罗河病毒和登革热2型病毒性脑炎的空斑试验培养检测法。
Virol J. 2006 Jan 6;3:2. doi: 10.1186/1743-422X-3-2.
4
Comparison of six commercial DNA extraction kits for recovery of cytomegalovirus DNA from spiked human specimens.六种用于从加标人标本中回收巨细胞病毒DNA的商用DNA提取试剂盒的比较。
J Clin Microbiol. 2000 Oct;38(10):3860-3. doi: 10.1128/JCM.38.10.3860-3863.2000.
5
Rapid detection of respiratory viruses by shell vial assay using simultaneous culture of HEp-2, LLC-MK2, and MDCK cells in a single vial.通过在单个小瓶中同时培养人喉表皮样癌细胞（HEp-2）、绿猴肾细胞（LLC-MK2）和犬肾细胞（MDCK），利用空斑试验快速检测呼吸道病毒。
J Clin Microbiol. 1999 Jul;37(7):2346-7. doi: 10.1128/JCM.37.7.2346-2347.1999.
6
Laboratory diagnosis of respiratory virus infections in 24 hours by utilizing shell vial cultures.利用空斑试验培养法在24小时内对呼吸道病毒感染进行实验室诊断。
J Clin Microbiol. 1997 Aug;35(8):2165-7. doi: 10.1128/jcm.35.8.2165-2167.1997.
7
Evaluation of the Digene Hybrid Capture System for detection and quantitation of human cytomegalovirus viremia in human immunodeficiency virus-infected patients.评估Digene杂交捕获系统用于检测和定量人类免疫缺陷病毒感染患者的人巨细胞病毒血症。
J Clin Microbiol. 1996 Dec;34(12):2959-62. doi: 10.1128/jcm.34.12.2959-2962.1996.
本文引用的文献
1
Rapid detection of cytomegalovirus in MRC-5 cells inoculated with urine specimens by using low-speed centrifugation and monoclonal antibody to an early antigen.通过低速离心和使用针对早期抗原的单克隆抗体，快速检测接种尿液标本的MRC-5细胞中的巨细胞病毒。
J Clin Microbiol. 1984 Jun;19(6):917-9. doi: 10.1128/jcm.19.6.917-919.1984.
2
Rapid detection of influenza virus by shell vial assay with monoclonal antibodies.利用单克隆抗体通过空斑试验快速检测流感病毒。
J Clin Microbiol. 1986 Oct;24(4):677-9. doi: 10.1128/jcm.24.4.677-679.1986.
3
Detection of cytomegalovirus infections in specimens other than urine by the shell vial assay and conventional tube cell cultures.通过空斑试验和传统的试管细胞培养法检测尿液以外标本中的巨细胞病毒感染。
J Clin Microbiol. 1987 May;25(5):755-7. doi: 10.1128/jcm.25.5.755-757.1987.
4
Rapid detection of herpes simplex virus in clinical specimens by centrifugation and immunoperoxidase staining.通过离心和免疫过氧化物酶染色快速检测临床标本中的单纯疱疹病毒。
J Clin Microbiol. 1986 Apr;23(4):683-6. doi: 10.1128/jcm.23.4.683-686.1986.
5
Monoclonal antibody for rapid laboratory detection of cytomegalovirus infections: characterization and diagnostic application.用于巨细胞病毒感染快速实验室检测的单克隆抗体：特性与诊断应用
Mayo Clin Proc. 1985 Sep;60(9):577-85. doi: 10.1016/s0025-6196(12)60979-3.
6
Detection and serotyping of herpes simplex virus in MRC-5 cells by use of centrifugation and monoclonal antibodies 16 h postinoculation.接种后16小时，通过离心和单克隆抗体在MRC-5细胞中检测单纯疱疹病毒并进行血清分型。
J Clin Microbiol. 1985 Jan;21(1):29-32. doi: 10.1128/jcm.21.1.29-32.1985.
7
Use of murine monoclonal antibodies for laboratory diagnosis of varicella-zoster virus infection.鼠单克隆抗体在水痘-带状疱疹病毒感染实验室诊断中的应用。
J Clin Microbiol. 1988 Sep;26(9):1623-5. doi: 10.1128/jcm.26.9.1623-1625.1988.
8
Evaluation of immunofluorescent reagents, centrifugation, and conventional cultures for the diagnosis of adenovirus infection.评估免疫荧光试剂、离心法和传统培养法用于腺病毒感染的诊断。
Diagn Microbiol Infect Dis. 1989 Sep-Oct;12(5):407-11. doi: 10.1016/0732-8893(89)90111-9.
9
Effect of age of shell vial monolayers on detection of cytomegalovirus from urine specimens.空斑试验单层细胞的年龄对从尿液标本中检测巨细胞病毒的影响。
J Clin Microbiol. 1989 Sep;27(9):2107-9. doi: 10.1128/jcm.27.9.2107-2109.1989.
10
Detection of respiratory syncytial virus in nasopharyngeal secretions by shell vial technique.采用空斑小室技术检测鼻咽分泌物中的呼吸道合胞病毒。
J Clin Microbiol. 1991 Mar;29(3):463-5. doi: 10.1128/jcm.29.3.463-465.1991.
Zotero 插件