以3500×g离心塑料培养瓶对临床标本中病毒检测的影响。
Effect of centrifuging shell vials at 3,500 x g on detection of viruses in clinical specimens.
作者信息
Engler H D, Selepak S T
机构信息
Clinical Pathology Department, Warren Grant Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892-0001.
出版信息
J Clin Microbiol. 1994 Jun;32(6):1580-2. doi: 10.1128/jcm.32.6.1580-1582.1994.
Abstract
An increase in shell vial centrifugation force to 3,500 x g and a concomitant reduction in spin time to 15 min did not decrease the sensitivity of detecting viruses in clinical specimens compared with the accepted practice of using 700 x g for 40 min. No damage to the cell monolayer (ML) at the higher g force was observed. Toxicity to the ML is decreased with the shorter spin, probably because of reduced time of contact between the specimen and the ML.
摘要
与采用700×g离心40分钟的公认做法相比，将空斑离心力提高到3500×g并同时将离心时间缩短至15分钟，并未降低临床标本中病毒检测的灵敏度。在较高的重力下未观察到细胞单层（ML）受损。较短的离心时间降低了对细胞单层的毒性，这可能是因为标本与细胞单层的接触时间减少了。
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