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使用细胞离心涂片的荧光抗体染色结合细胞培养来直接检测呼吸道病毒。

Use of fluorescent-antibody staining of cytocentrifuge-prepared smears in combination with cell culture for direct detection of respiratory viruses.

作者信息

Doing K M, Jerkofsky M A, Dow E G, Jellison J A

机构信息

Department of Microbiology, Biochemistry and Molecular Biology, University of Maine, Orono 04469, USA.

出版信息

J Clin Microbiol. 1998 Jul;36(7):2112-4. doi: 10.1128/JCM.36.7.2112-2114.1998.

DOI:10.1128/JCM.36.7.2112-2114.1998
PMID:9650977
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC104993/
Abstract

Over a 3-year period, 1,003 respiratory samples were collected and examined for selected respiratory viruses with cytocentrifuged prepared smears stained with fluorescently labeled antibodies (IFA) in conjunction with cell culture. IFA results were compared with results obtained by cell culture. Viruses were isolated or detected by direct means in 401 samples. Agreement between culture and IFA was 90%.

摘要

在3年期间，共采集了1003份呼吸道样本，并通过细胞离心涂片，用荧光标记抗体（免疫荧光法）染色，结合细胞培养，对选定的呼吸道病毒进行检测。将免疫荧光法的结果与细胞培养获得的结果进行比较。在401份样本中通过直接方法分离或检测到了病毒。培养法与免疫荧光法的一致性为90%。

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本文引用的文献

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Comparison of rapid diagnostic techniques for respiratory syncytial and influenza A virus respiratory infections in young children.幼儿呼吸道合胞病毒和甲型流感病毒呼吸道感染快速诊断技术的比较
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Detection of respiratory syncytial, parainfluenza type 2, and adenovirus antigens by radioimmunoassay and enzyme immunoassay on nasopharyngeal specimens from children with acute respiratory disease.采用放射免疫测定法和酶免疫测定法对患有急性呼吸道疾病儿童的鼻咽标本进行呼吸道合胞病毒、2型副流感病毒和腺病毒抗原检测。
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Comparison of HEp-2 cell culture and Abbott respiratory syncytial virus enzyme immunoassay.人喉表皮样癌细胞（HEp-2）细胞培养与雅培呼吸道合胞病毒酶免疫测定的比较。
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