Hanff G, Abrahamsson S O
Department of Hand Surgery, University Hospital, Malmö, Sweden.
J Hand Surg Br. 1996 Oct;21(5):642-6. doi: 10.1016/s0266-7681(96)80149-5.
Expanded polytetrafluoroethylene (e-PTFE) may be used as a barrier to reduce formation of restrictive adhesions following tendon surgery within the flexor tendon sheath region. In order to assess its effects on the cellular activity of healing tendons, synthesis and contents of matrix components and synthesis of DNA were compared in divided and sutured flexor tendons that either had been covered with e-PTFE membranes or with sham-operated tendon sheaths in 30 rabbits. At intervals of up to 12 weeks segments of the tendon repair sites were harvested, placed in wells and labelled with 35S-sulphate, 3H-proline and 3H-thymidine during short-term culture in vitro. Adverse tissue reactions and tendon disruptions were not observed at harvest. At each time interval synthesis of matrix components and DNA and contents of protein and collagen in the repaired tendon segments were similar in the two groups. Measured over all intervals, synthesis of non-collagen protein and contents of protein and collagen were lower in the e-PTFE groups. These results show that reconstruction of flexor tendon sheaths with e-PTFE membranes may not significantly impair cell proliferation but to some extent may reduce protein synthesis of healing tendons.
膨体聚四氟乙烯（e-PTFE）可用作屏障，以减少屈肌腱鞘区域肌腱手术后限制性粘连的形成。为了评估其对愈合肌腱细胞活性的影响，在30只兔子中，对分别用e-PTFE膜覆盖或假手术肌腱鞘处理的离断并缝合的屈肌腱，比较其基质成分的合成及含量和DNA的合成情况。在长达12周的时间间隔内，采集肌腱修复部位的组织块，置于培养孔中，并在体外短期培养期间用35S-硫酸盐、3H-脯氨酸和3H-胸腺嘧啶核苷进行标记。取材时未观察到不良组织反应和肌腱断裂。在每个时间间隔，两组修复肌腱段中基质成分和DNA的合成以及蛋白质和胶原蛋白的含量相似。在所有时间间隔内测量，e-PTFE组中非胶原蛋白的合成以及蛋白质和胶原蛋白的含量较低。这些结果表明，用e-PTFE膜重建屈肌腱鞘可能不会显著损害细胞增殖，但在一定程度上可能会减少愈合肌腱的蛋白质合成。
