Abrahamsson S O
Department of Hand Surgery, University Hospital, Malmö, Sweden.
J Hand Surg Br. 1996 Jun;21(3):299-302. doi: 10.1016/s0266-7681(05)80188-3.
In order to investigate the cellular effects of exposure to air during surgery and to compare the effects of simultaneous irrigation with physiological saline, the deep flexor tendons of both forepaws of 12 rabbits were surgically exposed. In one experiment, the extent of surgical exposure and, in a second experiment, the time of exposure was evaluated. Treated segments of the flexor tendons were collected and labelled in vitro for determination of the ability to synthesize DNA, proteoglycan, collagen and non-collagen protein. With increasing surgical exposure in vivo, an increasing rate of cellular proliferation was observed in segments of the exposed deep flexor tendons examined in vitro. Synthesis of matrix components and the rate of cellular proliferation were reduced by 50% after 40 to 100 minutes of exposure to air and by nearly 100% after 120 minutes of exposure. In contrast, irrigated tendons retained their cellular capacity to proliferate.
为了研究手术过程中暴露于空气中对细胞的影响，并比较同时用生理盐水冲洗的效果，对12只兔子双前爪的屈指深肌腱进行手术暴露。在一个实验中评估手术暴露的程度，在另一个实验中评估暴露时间。收集处理过的屈肌腱节段并在体外进行标记，以测定合成DNA、蛋白聚糖、胶原蛋白和非胶原蛋白的能力。随着体内手术暴露时间的增加，体外检查的暴露屈指深肌腱节段中细胞增殖速率增加。暴露于空气中40至100分钟后，基质成分的合成和细胞增殖速率降低了50%，暴露120分钟后几乎降低了100%。相比之下，冲洗过的肌腱保留了其细胞增殖能力。
