López-Moya J J, López-Abella D, Díaz-Rúiz J R, Martinez-Garcia B, Gáborjányi R
Centro de Investigaciones Biológicas, C. Velázquez, Madrid, Spain.
Z Naturforsch C J Biosci. 1997 May-Jun;52(5-6):391-5. doi: 10.1515/znc-1997-5-618.
Three Hungarian (no. 2, 4 and 9), and a Moldavian (K) plum pox virus isolates were compared with a characterized Spanish isolate (5.15) by RT-PCR, ELISA, dot-blot and Western blot analysis. Monoclonal antibodies prepared against the external, intermediate and internal sequences of the coat protein of the Spanish isolate were able to differentiate the four isolates. Hungarian isolate No. 2 proved to be serologically identical to the Spanish isolate, while No. 4 showed appreciable differences and No. 9 could be recognized only by the monoclonal antibodies representing the intermedial and internal parts of the coat protein. K isolate showed a more distant relationship to other isolates. Our experiment provided the first demonstration of the presence of D type isolates in Hungary.
通过逆转录聚合酶链反应（RT-PCR）、酶联免疫吸附测定（ELISA）、斑点印迹法和蛋白质免疫印迹分析，将3株匈牙利李痘病毒分离株（编号2、4和9）和1株摩尔多瓦李痘病毒分离株（K）与一株特征明确的西班牙分离株（5.15）进行了比较。针对西班牙分离株外壳蛋白外部、中间和内部序列制备的单克隆抗体能够区分这4株分离株。结果表明，匈牙利分离株2在血清学上与西班牙分离株相同，而分离株4表现出明显差异，分离株9只能被代表外壳蛋白中间和内部部分的单克隆抗体识别。K分离株与其他分离株的关系更为疏远。我们的实验首次证明匈牙利存在D型分离株。
