Jauniaux E, Gulbis B, Schandene L, Collette J, Hustin J
Academic Department of Obstetrics and Gynaecology, University College London Medical School, UK.
Mol Hum Reprod. 1996 Apr;2(4):239-43. doi: 10.1093/molehr/2.4.239.
Interleukin-6 (IL-6) distribution was investigated in coelomic fluid, amniotic fluid, maternal serum, decidua and placental villous tissue collected from 16 normal pregnancies between 7 and 12 weeks of gestation. IL-6 levels in fluids and tissues were measured by an immunoenzymatic assay and mouse monoclonal antibodies specific for IL-6 were used to localize immunohistochemically IL-6 in decidual and placental tissue. IL-6 was detected in all samples of coelomic and amniotic fluids and in most extracts of placental and decidual tissues. IL-6 concentration was significantly (P < 0.005) higher in the coelomic fluid than in amniotic fluid and was positively correlated with gestational age. Immunostaining for IL-6 was present in both syncytiotrophoblast and extra-villous trophoblast. IL-6 was in significantly (P < 0.001) higher concentration in decidual than in placental tissues. These data indicate that IL-6 is normally present in coelomic and amniotic fluids of early pregnancy and that IL-6 concentrations mainly result from the accumulation of trophoblastic-derived IL-6. During the first trimester IL-6 could play a role in tissue remodelling associated with placentation but also in the haematopoiesis function of the secondary yolk sac and in the generation of new vessels in placental villous tissue.
对16例妊娠7至12周的正常孕妇的体腔液、羊水、母血清、蜕膜和胎盘绒毛组织中的白细胞介素-6（IL-6）分布进行了研究。通过免疫酶测定法测量体液和组织中的IL-6水平，并使用针对IL-6的小鼠单克隆抗体对蜕膜和胎盘组织中的IL-6进行免疫组织化学定位。在所有体腔液和羊水样本以及大多数胎盘和蜕膜组织提取物中均检测到IL-6。体腔液中IL-6浓度显著高于羊水（P<0.005），且与孕周呈正相关。合体滋养层细胞和绒毛外滋养层细胞均有IL-6免疫染色。蜕膜中IL-6浓度显著高于胎盘组织（P<0.001）。这些数据表明，IL-6正常存在于早孕的体腔液和羊水中，且IL-6浓度主要源于滋养层来源的IL-6的积累。在妊娠早期，IL-6可能在与胎盘形成相关的组织重塑中发挥作用，也可能在次级卵黄囊的造血功能以及胎盘绒毛组织新血管的生成中发挥作用。
