Adeyefa C A
Department of Veterinary Medicine, University of Ibadan, Nigeria.
Rev Elev Med Vet Pays Trop. 1996;49(4):295-8.
The rapid diagnosis of African horse sickness (AHS) during the incubation period using virus antigens in peripheral blood mononuclear cells (PBMC) and red blood cells (RBC) in a sandwich indirect enzyme-linked immunosorbent assay (ELISA) is reported. PMBC consistently gave higher positive ELISA results than RBC from blood collected during viraemia from clinically affected horses. The potential of the method described for wider application in rapid diagnosis and virus surveillance in susceptible equine populations, particularly in AHS-free and in enzootic areas, for effective control strategies is highlighted.
据报道，采用夹心间接酶联免疫吸附测定法（ELISA），利用外周血单核细胞（PBMC）和红细胞（RBC）中的病毒抗原，可在潜伏期快速诊断非洲马瘟（AHS）。对于临床感染马匹在病毒血症期间采集的血液，PBMC的ELISA阳性结果始终高于RBC。文中强调了所描述方法在易感马群中进行快速诊断和病毒监测方面更广泛应用的潜力，特别是在无AHS地区和疫区，这对于有效的防控策略具有重要意义。
