来自紫色非硫细菌沼泽红假单胞菌7号菌株的磷酸烯醇式丙酮酸羧化酶基因的结构与功能分析
Structural and functional analysis of the phosphoenolpyruvate carboxylase gene from the purple nonsulfur bacterium Rhodopseudomonas palustris No. 7.
作者信息
Inui M, Dumay V, Zahn K, Yamagata H, Yukawa H
机构信息
Research Institute of Innovative Technology for the Earth, Soraku, Kyoto, Japan.
出版信息
J Bacteriol. 1997 Aug;179(15):4942-5. doi: 10.1128/jb.179.15.4942-4945.1997.
Abstract
The ppc gene, encoding phosphoenolpyruvate carboxylase (PEPC), from Rhodopseudomonas palustris No. 7 was cloned and sequenced. Primer extension analysis identified a transcriptional start site 42 bp upstream of the ppc initiation codon. An R. palustris No. 7 PEPC-deficient strain showed a slower doubling time compared with the wild-type strain either anaerobically in the light or aerobically in the dark, when pyruvate was used as a carbon source.
摘要
克隆并测序了来自沼泽红假单胞菌7号菌株的ppc基因，该基因编码磷酸烯醇式丙酮酸羧化酶（PEPC）。引物延伸分析确定了ppc起始密码子上游42 bp处的一个转录起始位点。当以丙酮酸作为碳源时，沼泽红假单胞菌7号PEPC缺陷型菌株在光照厌氧条件下或黑暗需氧条件下，与野生型菌株相比，其倍增时间较慢。
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