Roberts D M
Cytotechnology Program, Indiana University School of Medicine, Indianapolis, USA.
Acta Cytol. 1997 Jul-Aug;41(4):1008-14. doi: 10.1159/000332781.
To determine the effects of smokeless tobacco (snuff) on the epithelium of the oral cavity.
Cytologic smears were taken from the oral cavities of 22 snuff users and 19 control subjects. The samples were stained with the Papanicolaou method and accessed for cellularity, anucleated cells, micronuclei and broken egg nuclei.
An average of 7,624 cells per slide were found in the user group and 1,348 in the control. An average of 3,619 (47.5%) anucleated cells in the user group and 33.1 (2.5%) in the control were found per slide. Users with lesions had a significantly higher rate of anucleation than those without lesions. A total of 216 micronuclei were found in the user group and 46 in the control group. The broken egg nucleus was commonly found in both groups. The broken egg phenomenon is described as a nucleus that is in two portions and connected by a thin band of Feulgen-negative material.
The regular use of snuff causes loss of cell cohesion, hyperkeratosis and an increased incidence of micronuclei. The relationship of these conditions to the development of oral cancer is unclear. The most likely anomaly that may be associated with carcinogenesis is the micronucleus.
确定无烟烟草（鼻烟）对口腔上皮的影响。
从22名鼻烟使用者和19名对照者的口腔中采集细胞学涂片。样本采用巴氏染色法染色，并对细胞数量、无核细胞、微核和碎核进行评估。
使用者组每张玻片平均发现7624个细胞，对照组为1348个。使用者组每张玻片平均发现3619个（47.5%）无核细胞，对照组为33.1个（2.5%）。有病变的使用者无核化率明显高于无病变者。使用者组共发现216个微核，对照组为46个。碎核在两组中均常见。碎核现象是指细胞核分成两部分，由一条Feulgen阴性物质的细带相连。
经常使用鼻烟会导致细胞黏附丧失、角化过度和微核发生率增加。这些情况与口腔癌发生发展的关系尚不清楚。最可能与致癌作用相关的异常是微核。
