de Geus J-L, Wambier L-M, Loguercio A-D, Reis A
Rua Joaquim Sá - Dionísio Torres, Fortaleza, CE, 60135-218, Brasil,
Med Oral Patol Oral Cir Bucal. 2019 Mar 1;24(2):e145-e155. doi: 10.4317/medoral.22846.
The aim of this systematic review was to evaluate the frequency of micronuclei or other DNA damage in the oral mucosa of adults that have smokeless tobacco habits compared to adults that not have these habits.
We searched PubMed, Scopus, Web of Science, LILACS, BBO and Cochrane Library and SIGLE. We also surveyed gray literature. We included only clinical trials that compare the frequency of micronuclei or other DNA damage in the oral mucosa of adults that have smokeless tobacco habits compared to adults that not have these habits. Quality assessments of the selected trials were evaluated by two independent reviewers, using the Effective Public Health Practice Project - (EPHPP) with modifications.
After the database screening and removal of duplicates, 2574 studies were identified. After title screening, 172 studies remained, and this number was reduced to 25 after careful examination of the abstracts. The standardized mean difference of the frequency of micronuclei between groups was 1.88, with a 95% confidence interval of 1.40 to 2.36 (p < 0.00001). In all analyses heterogeneity was detected.
Despite the heterogeneity of studies, the frequency of micronuclei was significant bigger in adults who have the smokeless tobacco habit when compared to those not have this habit. The same occurred with the frequency of binucleated cells, karyolisis and karyorrhexis.
本系统评价的目的是评估有无烟烟草使用习惯的成年人与无此习惯的成年人相比，口腔黏膜中微核或其他DNA损伤的频率。
我们检索了PubMed、Scopus、Web of Science、LILACS、BBO、Cochrane图书馆和SIGLE。我们还调查了灰色文献。我们仅纳入了比较有无烟烟草使用习惯的成年人与无此习惯的成年人口腔黏膜中微核或其他DNA损伤频率的临床试验。由两名独立 reviewers 使用经修改的有效公共卫生实践项目（EPHPP）对所选试验进行质量评估。
在数据库筛选和去除重复项后，共识别出2574项研究。经过标题筛选，剩下172项研究，在仔细审查摘要后，这一数字降至25项。两组间微核频率的标准化平均差为1.88，95%置信区间为1.40至2.36（p < 0.00001）。在所有分析中均检测到异质性。
尽管研究存在异质性，但有无烟烟草使用习惯的成年人的微核频率显著高于无此习惯的成年人。双核细胞、核溶解和核碎裂的频率也有同样情况。
