MacDonald S L, McLaughlin L F, MacGregor I R, Pepper D S
Scottish National Blood Transfusion Service, Headquarters Unit Laboratory, Edinburgh, UK.
Transfus Med. 1991 Mar;1(1):17-23. doi: 10.1111/j.1365-3148.1991.tb00004.x.
Aqueous solutions of stroma-free human haemoglobin are being evaluated as potential oxygen-carrying resuscitation fluids. There are indications, however, that such solutions may produce toxic side-effects in vivo. Stroma-free haemoglobin solution produced a 50% fall in mean arterial pressure when infused into a small animal model despite containing very low levels of non-haem protein and phospholipid contaminants. This effect was not produced by haemoglobin solutions after extensive dialysis. Red cell-derived adenine nucleotides were found to be present in concentrations high enough to cause such a response (80-85 micrograms/ml). We have developed a chromatographic assay capable of predicting hypotension in our animal model and consider that the complete absence of adenine nucleotides must be confirmed in all studies concerning the possible toxic side-effects of stroma-free haemoglobin solutions.
无基质人血红蛋白水溶液正在作为潜在的携氧复苏液进行评估。然而，有迹象表明，这类溶液在体内可能产生毒性副作用。尽管无基质血红蛋白溶液中所含的非血红蛋白和磷脂污染物水平极低，但将其注入小动物模型时，平均动脉压会下降50%。经过广泛透析后的血红蛋白溶液不会产生这种效应。发现红细胞衍生的腺嘌呤核苷酸浓度高到足以引起这种反应（80 - 85微克/毫升）。我们开发了一种色谱分析方法，能够预测我们动物模型中的低血压情况，并认为在所有关于无基质血红蛋白溶液可能产生的毒性副作用的研究中，必须确认完全不存在腺嘌呤核苷酸。
