Wlaschek M, Wenk J, Brenneisen P, Briviba K, Schwarz A, Sies H, Scharffetter-Kochanek K
Department of Dermatology, University of Cologne, Germany.
FEBS Lett. 1997 Aug 18;413(2):239-42. doi: 10.1016/s0014-5793(97)00919-8.
Ultraviolet (UV) A irradiation of human dermal fibroblasts elicits an increase in specific mRNA amounts and bioactivities of the cytokines IL-1alpha, IL-1beta, and IL-6. These effects are enhanced in deuterium oxide-based medium and are diminished in the presence of non-toxic concentrations of sodium azide. Furthermore, generating singlet oxygen outside the cells by irradiation of rose bengal-coated resin particles with visible light (lambda > 450 nm) results in the induction of interstitial collagenase, IL-1 and IL-6, similar to the response observed with UVA irradiation. These observations suggest that singlet oxygen is an early intermediate in the signaling pathway of IL-1 and IL-6 mediating UVA induction of interstitial collagenase (E.C. 3.4.24.7). Furthermore, singlet oxygen appears to initiate this complex UV response at the cell membrane.
紫外线A（UVA）照射人皮肤成纤维细胞会引发细胞因子IL-1α、IL-1β和IL-6的特定mRNA量及生物活性增加。在基于重水的培养基中这些效应会增强，而在无毒浓度的叠氮化钠存在时则会减弱。此外，通过用可见光（波长>450 nm）照射孟加拉玫瑰红包被的树脂颗粒在细胞外产生单线态氧，会导致间质胶原酶、IL-1和IL-6的诱导，这与UVA照射所观察到的反应相似。这些观察结果表明单线态氧是介导UVA诱导间质胶原酶（E.C. 3.4.24.7）的IL-1和IL-6信号通路中的早期中间体。此外，单线态氧似乎在细胞膜处引发这种复杂的紫外线反应。
