Scharffetter-Kochanek K, Wlaschek M, Briviba K, Sies H
Hautklinik, Heinrich-Heine-Universität Düsseldorf, Germany.
FEBS Lett. 1993 Oct 4;331(3):304-6. doi: 10.1016/0014-5793(93)80357-z.
Singlet oxygen generated in a dark reaction by thermodissociation of an endoperoxide (NDPO2) elicits an increase in mRNA of interstitial collagenase (MMP-1) in cultured human fibroblasts. The effect is enhanced in deuterium oxide-based medium and is abolished in the presence of non-toxic doses of sodium azide. In contrast, the mRNA level of the tissue inhibitor of metalloproteinases (TIMP-1) remains unaltered under these experimental conditions. These observations support the suggestion that an unbalanced synthesis of collagenase and TIMP reported to occur following UV-A irradiation or during inflammatory conditions may be mediated by singlet oxygen.
内过氧化物（NDPO2）热解离在暗反应中产生的单线态氧可使培养的人成纤维细胞中间质胶原酶（MMP-1）的mRNA增加。在基于重水的培养基中该效应增强，而在无毒剂量的叠氮化钠存在时则被消除。相比之下，金属蛋白酶组织抑制剂（TIMP-1）的mRNA水平在这些实验条件下保持不变。这些观察结果支持了以下观点：据报道在UV-A照射后或炎症状态下发生的胶原酶和TIMP合成失衡可能由单线态氧介导。
