Department of Physics of Materials, Faculty of Sciences, Autonomous University of Madrid, Madrid, Spain; Formerly at Aarhus Institute of Advanced Studies (AIAS)/Department of Chemistry, Aarhus University, Aarhus, Denmark.
Redox Biol. 2017 Oct;13:39-59. doi: 10.1016/j.redox.2017.05.011. Epub 2017 May 25.
Molecular oxygen (O) displays very interesting properties. Its first excited state, commonly known as singlet oxygen (O), is one of the so-called Reactive Oxygen Species (ROS). It has been implicated in many redox processes in biological systems. For many decades its role has been that of a deleterious chemical species, although very positive clinical applications in the Photodynamic Therapy of cancer (PDT) have been reported. More recently, many ROS, and also O, are in the spotlight because of their role in physiological signaling, like cell proliferation or tissue regeneration. However, there are methodological shortcomings to properly assess the role of O in redox biology with classical generation procedures. In this review the direct optical excitation of O to produce O will be introduced, in order to present its main advantages and drawbacks for biological studies. This photonic approach can provide with many interesting possibilities to understand and put to use ROS in redox signaling and in the biomedical field.
分子氧（O）具有非常有趣的性质。其第一激发态，通常称为单线态氧（O），是所谓的活性氧物种（ROS）之一。它已被牵涉到生物系统中的许多氧化还原过程中。几十年来，它一直是一种有害的化学物质，尽管在癌症的光动力疗法（PDT）中有非常积极的临床应用已有报道。最近，许多 ROS，甚至 O，由于它们在生理信号传递中的作用，如细胞增殖或组织再生，而成为关注焦点。然而，使用经典的生成方法来正确评估 O 在氧化还原生物学中的作用存在方法学上的缺陷。在这篇综述中，将介绍直接用光激发 O 产生 O 的方法，以介绍其在生物学研究中的主要优点和缺点。这种光子方法可以为理解和利用 ROS 在氧化还原信号传递以及生物医学领域中的作用提供许多有趣的可能性。
