Guérin-Danan C, Andrieux C, Popot F, Charpilienne A, Vaissade P, Gaudichon C, Pedone C, Bouley C, Szylit O
Unité d'Ecologie et de Physiologie du Système Digestif, Equipe Métabolites Bactériens et Santé, Jouy en Josas, France.
J Pediatr Gastroenterol Nutr. 1997 Sep;25(3):281-9. doi: 10.1097/00005176-199709000-00007.
From 5 months of age, infants are progressively introduced to a variety of foods which influence the equilibrium of the intestinal microflora.
Thirty-five children age 10-18 months from 8 day care centers in France were studied. Fecal specimens were examined for their biochemical and microbiological criteria.
Bifidobacteria and Bacteroides belong to the predominant populations (9.7 and 8.6 log 10 cfu/g, respectively). The mean level of enterobacteria and enterococci were 8.0 and 7.8 log 10 cfu/g, respectively. Only 10% of the infants presented a lactobacilli amount above 6 log 10 cfu/g. Most feces had similar pH values (mean 6.4), percentage of water (mean 76.4%), and short chain fatty acid, ammonia and lactic acid concentrations (means 77, 6.7 and 2.3 mumol/g, respectively). beta-galactosidase had the highest activity (106 IU/g of protein) and nitroreductase, the lowest (0.1 IU/g of protein). alpha-glucosidase and nitrate reductase showed intermediate values of 17 and 4 IU/g of protein, respectively. With the exception of 4 infants, beta-glucosidase and beta-glucuronidase values were low (4 and 2 IU/g of protein). Age and day care center were not significant factors for most parameters studied, except that rotavirus was related to day care center, with detection in 5 infants from the same center.
Many biochemical parameters were comparable to those found in adults, with the exception of ammonia concentration and beta-galactosidase activity. The fecal bacterial profile was different than in adults, with more Bifidobacteria than Bacteroides and higher levels of facultative anaerobes. One infant suffering from gastroenteritis had distinctive biochemical and bacterial parameters.
从5个月大开始，婴儿逐渐开始接触各种食物，这会影响肠道微生物群的平衡。
对来自法国8个日托中心的35名10 - 18个月大的儿童进行了研究。对粪便样本进行了生化和微生物学标准检测。
双歧杆菌和拟杆菌属于主要菌群（分别为9.7和8.6 log10 cfu/g）。肠杆菌和肠球菌的平均水平分别为8.0和7.8 log10 cfu/g。只有10%的婴儿乳酸杆菌数量高于6 log10 cfu/g。大多数粪便的pH值相似（平均6.4）、水分百分比（平均76.4%）以及短链脂肪酸、氨和乳酸浓度（分别平均为77、6.7和2.3 μmol/g）。β-半乳糖苷酶活性最高（106 IU/g蛋白质），硝基还原酶活性最低（0.1 IU/g蛋白质）。α-葡萄糖苷酶和硝酸盐还原酶的活性分别为17和4 IU/g蛋白质，处于中间值。除4名婴儿外，β-葡萄糖苷酶和β-葡萄糖醛酸酶的值较低（分别为4和2 IU/g蛋白质）。年龄和日托中心对大多数研究参数不是显著因素，但轮状病毒与日托中心有关，在同一中心的5名婴儿中检测到。
除氨浓度和β-半乳糖苷酶活性外，许多生化参数与成年人的相当。粪便细菌谱与成年人不同，双歧杆菌比拟杆菌多，兼性厌氧菌水平更高。一名患肠胃炎的婴儿有独特的生化和细菌参数。
