Erin N, Yegen B C, Oktay S
Marmara University, Medical Faculty, Department of Pharmacology, Haydarpasa-Istanbul, Turkey.
Peptides. 1997;18(6):893-8. doi: 10.1016/s0196-9781(97)00018-1.
The present study examined 1) oxidative stress and gastric lesions induced by thyrotropin releasing hormone (TRH) 2) The effect of a 5-hydroxytryptamine3 (5-HT3) receptor antagonist, ICS 205930 on protective effect of calcitonin on gastric lesions produced by TRH. Calcitonin (5 micrograms/10 microliter) was injected i.c.v. 10 min before TRH (10 micrograms/10 microliter, i.c.v.) injection or ICS 0.5 mg/kg, (i.p.) was given 60 min prior to calcitonin or TRH to rats. Ulcer index, lipid peroxidation (LP) and glutathione (GSH) levels were quantified 3 h after TRH injection in the stomach, liver and brain. TRH caused mucosal lesions (UI: 10.0 +/- 2.0 mm) without changing gastric GSH and LP. JCS did not alter the protective effect of calcitonin against TRH-induced lesions but attenuated. TRH-induced lesion formation. The oxidative effects of calcitonin or ICS were similar to TRH but both drugs attenuated gastric lesion formation. Hence, oxidative changes in tissues studied are not directly involved in TRH-induced lesions.
1）促甲状腺激素释放激素（TRH）诱导的氧化应激和胃损伤；2）5-羟色胺3（5-HT3）受体拮抗剂ICS 205930对降钙素对TRH所致胃损伤的保护作用的影响。在向大鼠脑室内注射TRH（10微克/10微升）前10分钟脑室内注射降钙素（5微克/10微升），或在降钙素或TRH注射前60分钟腹腔注射ICS 0.5毫克/千克。在注射TRH 3小时后，对胃、肝和脑的溃疡指数、脂质过氧化（LP）和谷胱甘肽（GSH）水平进行定量。TRH引起黏膜损伤（溃疡指数：10.0±2.0毫米），但未改变胃内GSH和LP水平。ICS未改变降钙素对TRH诱导损伤的保护作用，但减弱了TRH诱导的损伤形成。降钙素或ICS的氧化作用与TRH相似，但两种药物均减弱了胃损伤的形成。因此，所研究组织中的氧化变化与TRH诱导的损伤没有直接关系。
