Hebbachi A M, Seelaender M C, Baker B W, Gibbons G F
Oxford Lipid Metabolism Group, Metabolic Research Laboratory, Nuffield Department of Clinical Medicine, University of Oxford, Radcliffe Infirmary, Oxford OX2 6HE, U.K.
Biochem J. 1997 Aug 1;325 ( Pt 3)(Pt 3):711-9. doi: 10.1042/bj3250711.
Hepatocytes from rats fed a chow (control) diet or from rats fed a chow diet supplemented with either orotic acid (OA; 1%, w/w) or fish oil (FO; 20%, v/w) were maintained in culture for periods up to 48 h. during the first 24 h period, the low rates of output of very-low-density lipoprotein (VLDL)-associated triacylglycerol (TAG) and apolipoprotein B (apoB) in hepatocytes from the FO- and OA-fed animals were associated with significantly lower rates of intracellular TAG lipolysis and re-esterification. Most of the VLDL TAG secreted was mobilized via lipolysis of the intracellular TAG pool, but the proportion of VLDL TAG secreted via this route in cells from the FO-fed and OA-fed animals was decreased compared with that in the control-fed animals' cells. In the presence of exogenous oleate the inhibitory effect of OA feeding on VLDL apoB and TAG secretion persisted in the derived hepatocytes for up to 48 h following isolation. However, when oleate was absent no inhibitory effect on the secretion of TAG and apoB was observed between 24 and 48 h. Under these conditions the rate of intracellular TAG turnover returned to normal. The initial inhibitory effect of FO feeding on VLDL TAG and apoB secretion did not persist in the derived hepatocytes between 24 h and 48 h of culture in the presence of exogenous oleate. Although intracellular TAG lipolysis and VLDL TAG and apoB secretion rates appear to be positively correlated, a causal relationship has not been conclusively established.
将食用普通（对照）饮食的大鼠或食用添加了乳清酸（OA；1%，w/w）或鱼油（FO；20%，v/w）的普通饮食的大鼠的肝细胞在培养中维持长达48小时。在最初的24小时期间，来自喂食FO和OA的动物的肝细胞中极低密度脂蛋白（VLDL）相关三酰甘油（TAG）和载脂蛋白B（apoB）的低输出率与细胞内TAG脂解和再酯化率显著降低有关。分泌的大多数VLDL TAG是通过细胞内TAG池的脂解来动员的，但与对照喂养动物的细胞相比，来自喂食FO和OA的动物的细胞中通过该途径分泌的VLDL TAG的比例降低。在存在外源性油酸的情况下，喂食OA对VLDL apoB和TAG分泌的抑制作用在分离后的原代肝细胞中持续长达48小时。然而，当不存在油酸时，在24至48小时之间未观察到对TAG和apoB分泌的抑制作用。在这些条件下，细胞内TAG周转速率恢复正常。在存在外源性油酸的情况下，喂食FO对VLDL TAG和apoB分泌的初始抑制作用在培养24小时至48小时之间的原代肝细胞中并未持续。尽管细胞内TAG脂解与VLDL TAG和apoB分泌速率似乎呈正相关，但尚未最终确定因果关系。
