Nelson M A, Kang S, Braun E L, Crawford M E, Dolan P L, Leonard P M, Mitchell J, Armijo A M, Bean L, Blueyes E, Cushing T, Errett A, Fleharty M, Gorman M, Judson K, Miller R, Ortega J, Pavlova I, Perea J, Todisco S, Trujillo R, Valentine J, Wells A, Werner-Washburne M, Natvig D O
Department of Biology, University of New Mexico, Albuquerque 87131, USA.
Fungal Genet Biol. 1997 Jun;21(3):348-63. doi: 10.1006/fgbi.1997.0986.
In the Neurospora Genome Project at the University of New Mexico, expressed sequence tags (ESTs) corresponding to three stages of the life cycle of the filamentous fungus Neurospora crassa are being analyzed. The results of a pilot project to identify expressed genes and determine their patterns of expression are presented. 1,865 partial complementary DNA (cDNA) sequences for 1,409 clones were determined using single-pass sequencing. Contig analysis allowed the identification of 838 unique ESTs and 156 ESTs present in multiple cDNA clones. For about 34% of the sequences, highly or moderately significant matches to sequences (of known and unknown function) in the NCBI database were detected. Approximately 56% of the ESTs showed no similarity to previously identified genes. Among genes with assigned function, about 43.3% were involved in metabolism, 32.9% in protein synthesis and 8.4% in RNA synthesis. Fewer were involved in defense (6%), cell signalling (3.4%), cell structure (3.4%) and cell division (2.6%).
在新墨西哥大学开展的粗糙脉孢菌基因组计划中，正在分析与丝状真菌粗糙脉孢菌生命周期三个阶段相对应的表达序列标签（ESTs）。本文展示了一个用于鉴定表达基因并确定其表达模式的试点项目的结果。使用单通道测序法确定了1409个克隆的1865个部分互补DNA（cDNA）序列。重叠群分析使得能够鉴定出838个独特的ESTs以及多个cDNA克隆中存在的156个ESTs。对于约34%的序列，在NCBI数据库中检测到与（已知和未知功能的）序列具有高度或中度显著的匹配。大约56%的ESTs与先前鉴定的基因没有相似性。在已确定功能的基因中，约43.3%参与代谢，32.9%参与蛋白质合成，8.4%参与RNA合成。参与防御（6%）、细胞信号传导（3.4%）、细胞结构（3.4%）和细胞分裂（2.6%）的基因较少。
