海分枝杆菌中光致变色所必需的crtB同源物：通过同源重组进行分离、表征及基因破坏。
A crtB homolog essential for photochromogenicity in Mycobacterium marinum: isolation, characterization, and gene disruption via homologous recombination.
作者信息
Ramakrishnan L, Tran H T, Federspiel N A, Falkow S
机构信息
Department of Microbiology and Immunology, Stanford University School of Medicine, California 94305-5402, USA.
出版信息
J Bacteriol. 1997 Sep;179(18):5862-8. doi: 10.1128/jb.179.18.5862-5868.1997.
Abstract
A gene essential for light-induced pigment production was isolated from the photochromogen Mycobacterium marinum by heterologous complementation of an M. marinum cosmid library in the nonchromogen Mycobacterium smegmatis. This gene is part of an operon and homologous to the Streptomyces griseus and Myxococcus xanthus crtB genes encoding phytoene synthase. Gene replacement at this locus was achieved via homologous recombination, demonstrating that its expression is essential for photochromogenicity. The ease of targeted gene disruption in this pathogenic Mycobacterium allows for the dissection of the molecular basis of mycobacterial pathogenesis.
摘要
通过在非产色分枝杆菌耻垢分枝杆菌中对海分枝杆菌黏粒文库进行异源互补，从光产色菌海分枝杆菌中分离出一个对光诱导色素产生至关重要的基因。该基因是一个操纵子的一部分，与编码八氢番茄红素合酶的灰色链霉菌和黄色黏球菌crtB基因同源。通过同源重组实现了该位点的基因替换，证明其表达对光产色性至关重要。在这种致病性分枝杆菌中进行靶向基因破坏的简便性，有助于剖析分枝杆菌发病机制的分子基础。
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