Chung L W, Coffey D S
Steroids. 1977 Aug;30(2):223-43. doi: 10.1016/0039-128x(77)90084-8.
A simple one-step procedure is described on the isolation of androgen glucuronides from various rat tissues. This procedure uses polyacrylamide gel electrophoresis, and permits a quantitative isolation of a single band containing the total androgen glucuronides without the contamination of free androgens and androgen sulfates. This procedure was used to determine the ability of various tissues of the rat to form androgen glucuronides directly when they were incubated with 1,2-[3H]-testosterone (0.1 micronm) in vitro. Of eleven organs studied, only the accessory sex organs (ventral prostate, seminal vesicle, and coagulating gland), liver, and kidney were capable of forming androgen glucuronides. At the end of a one-hour incubation period, approximately 1% of the total radiolabeled steroids in the prostatic tissue minces were in the form of glucuronide conjugates. The predominant androgen glucuronide formed in the accessory sex organs was 5 alpha -androstane-3 alpha, 17 beta-diol 17 beta-D-glucuronide. This is in contrast to the rat liver and kidney in which testosterone glucuronide was the predominant conjugate.
本文描述了一种从大鼠各种组织中分离雄激素葡萄糖醛酸苷的简单一步法。该方法采用聚丙烯酰胺凝胶电泳，可定量分离出包含总雄激素葡萄糖醛酸苷的单一谱带，且不会受到游离雄激素和雄激素硫酸盐的污染。该方法用于测定大鼠各种组织在体外与1,2-[³H] -睾酮（0.1微摩尔）孵育时直接形成雄激素葡萄糖醛酸苷的能力。在所研究的11个器官中，只有附属生殖器官（腹侧前列腺、精囊和凝固腺）、肝脏和肾脏能够形成雄激素葡萄糖醛酸苷。在1小时孵育期结束时，前列腺组织碎块中总放射性标记类固醇的约1%为葡萄糖醛酸苷结合物形式。在附属生殖器官中形成的主要雄激素葡萄糖醛酸苷是5α-雄甾烷-3α,17β-二醇17β-D-葡萄糖醛酸苷。这与大鼠肝脏和肾脏形成对比，在大鼠肝脏和肾脏中，睾酮葡萄糖醛酸苷是主要的结合物。
