由1-β-D-阿拉伯呋喃糖基胞嘧啶体外转化的仓鼠细胞中的C型RNA病毒。
C-type RNA virus from hamster cells transformed in vitro by 1-B-D-arabinofuranosylcytosine.
作者信息
Kouri R E, Kurtz S A, Vernon M L, Schechtman L M, Nicolson M, Klement V, Benedict W F
出版信息
Arch Virol. 1979;62(3):241-52. doi: 10.1007/BF01317556.
Evidence of type-C RNA viral activity in fetal hamster cells transformed in vitro by 1-B-D-arabinofuranosylcytosine (ara-C) after at least one in vivo passage is described. The virus possesses properties typical of other type-C RNA viruses, such as: a) morphology as determined with the electron microscope, b) presence of 70S RNA, c) enhanced expression following treatment with halogenated pyrimidines, d) group specific antigens of hamster type, and e) a buoyant density of 1.15 g per cm3. However, the virus particles are deficient in RNA-dependent DNA polymerase activity under conditions that easily detect Rauscher Leukemia virus and will infect neither hamster, rat, mouse, human nor rabbit cells. The possible role of this virus in chemical carcinogenesis of cultured hamster fetal cells is discussed.
本文描述了经1-β-D-阿拉伯呋喃糖基胞嘧啶（ara-C）体外转化的胎儿仓鼠细胞，在至少一次体内传代后具有C型RNA病毒活性的证据。该病毒具有其他C型RNA病毒的典型特性，例如：a）通过电子显微镜确定的形态；b）存在70S RNA；c）经卤代嘧啶处理后表达增强；d）仓鼠型群特异性抗原；e）浮力密度为每立方厘米1.15克。然而，在能轻易检测到劳氏肉瘤病毒的条件下，该病毒颗粒缺乏依赖RNA的DNA聚合酶活性，并且不会感染仓鼠、大鼠、小鼠、人类或兔细胞。本文还讨论了这种病毒在培养的仓鼠胎儿细胞化学致癌过程中可能发挥的作用。
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