Scherping S C, Schmidt C C, Georgescu H I, Kwoh C K, Evans C H, Woo S L
Department of Orthopaedic Surgery, University of Pittsburgh, PA 15213, USA.
Connect Tissue Res. 1997;36(1):1-8. doi: 10.3109/03008209709160209.
Growth factors have been shown to stimulate fibroblast proliferation during wound and ligament healing. In this study, we analyzed individual effects of eight growth factors in vitro on the proliferation of fibroblasts from the medial collateral (MCL) and anterior cruciate (ACL) ligaments of skeletally mature rabbits. We compared the proliferative response of growth factor-treated and nontreated fibroblasts of both ligaments. The growth-factor treated fibroblasts of the MCL and ACL were also compared. We found that the fibroblasts exposed to epidermal growth factor, basic fibroblast growth factor and platelet-derived growth factor-BB proliferated significantly more than untreated fibroblasts. Acidic fibroblast growth factor at a dose of 1.0 ng/ml caused significant increases in fibroblast proliferation only in the MCL. Transforming growth factor-beta 1, insulin-like growth factor-1, platelet-derived growth factor-AA, and interleukin-1 alpha did not significantly stimulate fibroblast proliferation. MCL fibroblasts generally did not proliferate significantly more than ACL fibroblasts with the exception of MCL fibroblasts exposed to the highest doses of basic fibroblast growth factor, acidic fibroblast growth factor and platelet-derived growth factor-BB. The data were also compared with those obtained earlier using fibroblasts from skeletally immature rabbits (Schmidt et al., JOR 1995). The proliferative response of both the MCL and the ACL fibroblasts was found to decrease with skeletal maturation. Thus, our findings suggest that animal age and fibroblast origin are important factors in determining the proliferative response to growth factors.
生长因子已被证明在伤口和韧带愈合过程中可刺激成纤维细胞增殖。在本研究中，我们分析了八种生长因子在体外对骨骼成熟兔内侧副韧带（MCL）和前交叉韧带（ACL）成纤维细胞增殖的单独影响。我们比较了生长因子处理组和未处理组的两条韧带成纤维细胞的增殖反应。我们还比较了MCL和ACL中经生长因子处理的成纤维细胞。我们发现，暴露于表皮生长因子、碱性成纤维细胞生长因子和血小板衍生生长因子-BB的成纤维细胞比未处理的成纤维细胞增殖明显更多。剂量为1.0 ng/ml的酸性成纤维细胞生长因子仅在MCL中显著增加成纤维细胞增殖。转化生长因子-β1、胰岛素样生长因子-1、血小板衍生生长因子-AA和白细胞介素-1α并未显著刺激成纤维细胞增殖。除了暴露于最高剂量碱性成纤维细胞生长因子、酸性成纤维细胞生长因子和血小板衍生生长因子-BB的MCL成纤维细胞外，MCL成纤维细胞通常不比ACL成纤维细胞增殖明显更多。我们还将这些数据与早期使用骨骼未成熟兔的成纤维细胞获得的数据进行了比较（施密特等人，《矫形外科学杂志》1995年）。发现MCL和ACL成纤维细胞的增殖反应均随骨骼成熟而降低。因此，我们的研究结果表明，动物年龄和成纤维细胞来源是决定对生长因子增殖反应的重要因素。
