Dennett C, Cleator G M, Klapper P E
Department of Pathological Sciences, University of Manchester, UK.
J Med Virol. 1997 Sep;53(1):1-3. doi: 10.1002/(sici)1096-9071(199709)53:1<1::aid-jmv1>3.0.co;2-4.
The incidence of herpes simplex virus type 1 (HSV-1) and herpes simplex virus type 2 (HSV-2) in herpes simplex encephalitis (HSE) was investigated using cerebrospinal fluid (CSF) samples from sixty-four cases of HSE. A polymerase chain reaction (PCR) employing primers flanking a region of the HSV thymidine kinase gene common to both HSV-1 and HSV-2 was used to detect HSV in the CSF. HSV-1 and HSV-2 were differentiated by digestion with restriction enzymes. Two enzymes were employed; Aval which cleaved only the HSV-2 gene product and Avall which cleaved only the HSV-1 gene product. Sixty-three cases of HSE were found to be due to HSV-1; one case due to HSV-2. These data confirm previous observations that HSV-2 is a rare cause of post-neonatal herpes encephalitis but indicates that a PCR procedure capable of detection of both viruses is essential for efficient diagnosis of HSE.
利用64例单纯疱疹性脑炎（HSE）患者的脑脊液（CSF）样本，对1型单纯疱疹病毒（HSV-1）和2型单纯疱疹病毒（HSV-2）在单纯疱疹性脑炎中的发病率进行了调查。采用聚合酶链反应（PCR），该反应使用位于HSV-1和HSV-2共有的HSV胸苷激酶基因区域两侧的引物，来检测脑脊液中的HSV。通过用限制性酶消化来区分HSV-1和HSV-2。使用了两种酶；Aval仅切割HSV-2基因产物，Avall仅切割HSV-1基因产物。发现63例HSE病例由HSV-1引起；1例由HSV-2引起。这些数据证实了之前的观察结果，即HSV-2是新生儿期后疱疹性脑炎的罕见病因，但表明一种能够检测两种病毒的PCR程序对于HSE的有效诊断至关重要。
