Shoji H, Koga M, Kusuhara T, Kaji M, Ayabe M, Hino H, Hondo R
First Department (Neurology) of Internal medicine, Kurume University School of Medicine, Japan.
J Neurol. 1994 Aug;241(9):526-30. doi: 10.1007/BF00873514.
Differentiation of herpes simplex virus (HSV) types 1 and 2 in cerebrospinal fluid of 17 patients with serologically diagnosed HSV encephalitis and meningitis or acute limbic encephalitis was determined by stringent hybridization of polymerase chain reaction--amplified DNAs. Ten of 17 patients were positive; six with HSV 1 isolates and four with HSV 2 isolates. We detected HSV type 1 in two cases of meningitis, although meningitis is generally thought to be caused by type 2. Additionally, HSV type 2 was found in one case of acute adult encephalitis, which is generally due to HSV type 1. HSV DNAs could be detected for over 1 month after onset, although our patients included several prolonged and recurrent cases. HSV DNA genomes were not detected in three cases of acute limbic encephalitis. Our study indicates that this method can be used for type differentiation in HSV CNS infections.
通过聚合酶链反应扩增DNA的严格杂交，对17例血清学诊断为单纯疱疹病毒（HSV）脑炎、脑膜炎或急性边缘叶脑炎患者的脑脊液进行HSV-1和HSV-2分型。17例患者中有10例呈阳性；6例分离出HSV-1，4例分离出HSV-2。我们在2例脑膜炎患者中检测到HSV-1，尽管一般认为脑膜炎通常由HSV-2引起。此外，在1例急性成人脑炎患者中发现了HSV-2，而急性成人脑炎一般由HSV-1引起。发病后1个多月仍可检测到HSV DNA，尽管我们的患者包括几例病程延长和复发的病例。3例急性边缘叶脑炎患者未检测到HSV DNA基因组。我们的研究表明，该方法可用于HSV中枢神经系统感染的分型。
