Sierra M A, Carrasco L, Gómez-Villamandos J C, Martin de las Mulas J, Méndez A, Jover A
Department of Pathology, Faculty of Veterinary Medicine, University of Córdoba, Spain.
J Comp Pathol. 1990 Apr;102(3):323-34. doi: 10.1016/s0021-9975(08)80021-7.
The role of pulmonary intravascular macrophages (PIM) in a viral infection has been studied by structural and ultrastructural methods with two strains of the African swine fever (ASF) virus: the virulent strain E70 and the attenuated strain E75. Pulmonary intravascular macrophages were the cells of the mononuclear phagocyte system of the lung most involved in the replication of strain E70, showing a marked cytopathic effect and necrosis. Concurrently, their size and number increased sharply. This stimulation of PIMs by the virulent strain of the ASF virus, together with the cytopathic effect, might be the cause of the abundant masses of cell debris found in septal capillaries and thus be related to the pulmonary oedema that characterizes the acute forms of the disease. With the attenuated strain of the ASF virus, stimulation of PIMs, as well as viral replication within them, was also intense, but the cytopathic effect was less. Later, in the course of the infection, the number of PIMs decreased sharply and then alveolar macrophages increased, bearing most of the viral replication and signalling the onset of a pneumonic process.
利用非洲猪瘟（ASF）病毒的两种毒株：强毒株E70和弱毒株E75，通过结构和超微结构方法研究了肺血管内巨噬细胞（PIM）在病毒感染中的作用。肺血管内巨噬细胞是肺单核吞噬细胞系统中最参与E70毒株复制的细胞，表现出明显的细胞病变效应和坏死。同时，它们的大小和数量急剧增加。ASF病毒强毒株对PIM的这种刺激，连同细胞病变效应，可能是在间隔毛细血管中发现大量细胞碎片的原因，因此与该疾病急性形式所特有的肺水肿有关。对于ASF病毒弱毒株，对PIM的刺激以及它们内部的病毒复制也很强烈，但细胞病变效应较小。后来，在感染过程中，PIM的数量急剧减少，然后肺泡巨噬细胞增加，承担了大部分病毒复制，并表明肺炎过程开始。
