Koenen F, Vanderhallen H, Papadopoulos O, Billinis C, Paschaleri-Papadopoulou E, Brocchi E, De Simone F, Carra E, Knowles N J
National Instituut voor Diergeneeskundig Onderzoek (NIDO-INRV), Ukkel, Belgium.
Res Vet Sci. 1997 May-Jun;62(3):239-44. doi: 10.1016/s0034-5288(97)90197-1.
The pathogenicity of two porcine encephalomyocarditis virus (EMCV) isolates for sows in gestation and young piglets was studied. One virus originated from a case of reproductive failure in pigs in Belgium and the other from a case of acute myocarditis in pigs in Greece. Sows in the mid-gestation period and one- to two-month old piglets were inoculated with each isolate. The molecular relationship between both isolates was studied by determining the nucleotide sequence located across the junction of the 1C and 1D capsid-coding genes. Antigenic analysis was performed using a panel of 35 monoclonal antibodies raised against an Italian field isolate of EMCV. All three approaches revealed differences between both isolates and also confirmed that there was no link between the two outbreaks of disease.
研究了两株猪脑心肌炎病毒（EMCV）分离株对妊娠母猪和仔猪的致病性。一株病毒源自比利时猪的一例繁殖障碍病例，另一株源自希腊猪的一例急性心肌炎病例。分别用每株分离株接种妊娠中期的母猪和1至2月龄的仔猪。通过测定位于衣壳编码基因1C和1D交界处的核苷酸序列，研究了两株分离株之间的分子关系。使用一组针对意大利EMCV野毒株产生的35种单克隆抗体进行抗原分析。所有三种方法均揭示了两株分离株之间的差异，也证实了这两起疾病暴发之间没有关联。
