Yoshida Y, Komatsu M, Ozeki A, Nango R, Tsukamoto I
Department of Food Science and Nutrition, Nara Women's University, Japan.
Biochim Biophys Acta. 1997 Aug 29;1336(2):180-6. doi: 10.1016/s0304-4165(97)00025-1.
The effects of ethanol on liver regeneration after partial hepatectomy were investigated. The injection of ethanol inhibited the increases in the activities of thymidylate synthase and thymidine kinase in regenerating rat liver at 24 h after partial hepatectomy in a dose-dependent manner, with a concomitant reduction in DNA content. Northern blot analysis showed that the inhibition of thymidylate synthase and thymidine kinase activities was caused by comparable decreases in their mRNA levels. The immunoblotting assay confirmed the protein levels of thymidylate synthase and thymidine kinase as proportional to the activity and mRNA levels. These findings suggest that ethanol inhibits DNA synthesis by the repression of mRNA levels of dTMP-synthesizing enzymes during liver regeneration.
研究了乙醇对部分肝切除术后肝脏再生的影响。注射乙醇以剂量依赖的方式抑制了部分肝切除术后24小时大鼠再生肝脏中胸苷酸合成酶和胸苷激酶活性的增加，同时DNA含量降低。Northern印迹分析表明，胸苷酸合成酶和胸苷激酶活性的抑制是由它们mRNA水平的相应降低引起的。免疫印迹分析证实胸苷酸合成酶和胸苷激酶的蛋白质水平与活性和mRNA水平成正比。这些发现表明，乙醇在肝脏再生过程中通过抑制dTMP合成酶的mRNA水平来抑制DNA合成。
