Shen H M, Chia S E, Ni Z Y, New A L, Lee B L, Ong C N
Department of Community, Occupational and Family Medicine, National University of Singapore.
Reprod Toxicol. 1997 Sep-Oct;11(5):675-80. doi: 10.1016/s0890-6238(97)00032-4.
The present study aims to evaluate oxidative DNA damage in human sperm and the association with cigarette smoking. The level of 8-hydroxydeoxyguanosine (8-OHdG) in sperm DNA, cotinine concentration in seminal plasma, and conventional seminal parameters such as semen volume, sperm density, viability, motility, and normal morphology were determined in 60 healthy subjects. It was found that the sperm DNA of smokers contained a significantly higher amount of 8-OHdG than that of nonsmokers (6.19 +/- 1.71 vs. 3.93 +/- 1.33 8-OHdG/10(5) dG, P < 0.001). The level of 8-OHdG in sperm DNA was also closely correlated to seminal cotinine concentration (r = 0.38, P < 0.05). These findings suggest that cigarette smoking enhances the extent of DNA damage in sperm. In contrast, no significant difference was observed for conventional parameters between smokers and nonsmokers, suggesting that the level of 8-OHdG in sperm may reflect the deleterious effect of cigarette smoking on sperm quality more accurately than conventional seminal parameters. Further investigation is required to understand the exact biologic and pathologic significance of oxidative damage to sperm DNA and the possibility of using 8-OHdG for the assessment of sperm quality.
本研究旨在评估人类精子中的氧化性DNA损伤及其与吸烟的关联。对60名健康受试者测定了精子DNA中的8-羟基脱氧鸟苷（8-OHdG）水平、精浆中的可替宁浓度以及常规精液参数，如精液量、精子密度、活力、运动能力和正常形态。结果发现，吸烟者精子DNA中的8-OHdG含量显著高于不吸烟者（6.19±1.71对3.93±1.33 8-OHdG/10(5) dG，P<0.001）。精子DNA中的8-OHdG水平也与精浆可替宁浓度密切相关（r = 0.38，P<0.05）。这些发现表明，吸烟会增加精子中DNA的损伤程度。相比之下，吸烟者和不吸烟者之间的常规参数未观察到显著差异，这表明精子中的8-OHdG水平可能比常规精液参数更准确地反映吸烟对精子质量的有害影响。需要进一步研究以了解精子DNA氧化损伤的确切生物学和病理学意义，以及使用8-OHdG评估精子质量的可能性。
