Bharadwaj M, Botten J, Torrez-Martinez N, Hjelle B
Department of Pathology, University of New Mexico School of Medicine, and University of New Mexico Cancer Research and Treatment Center, Albuquerque 87131-5301, USA.
Am J Trop Med Hyg. 1997 Sep;57(3):368-74. doi: 10.4269/ajtmh.1997.57.368.
Human hantavirus disease occurs throughout much of South America. The rodent hosts and the specific etiologic agent(s) are largely unknown, but many reported cases occurred within the habitation ranges of oryzomine rodents (rice rats). We have identified a genetically novel hantavirus (Rio Mamore virus [RM]) of the pygmy rice rat Oligoryzomys microtis in Bolivia. The complete sequence of the small (S) genome and the partial sequence of the medium (M) genome are described. This virus is closely related to the newly identified human pathogen Andes virus from Patagonia. To facilitate improved diagnosis of hantavirus infections in South America, we have expressed the complete nucleocapsid protein of RM in Escherichia coli and affinity-purified it for use in an ELISA and Western blot assays for antibodies to RM.
人类汉坦病毒病在南美洲大部分地区都有发生。啮齿动物宿主和具体的病原体大多未知，但许多报告病例发生在稻鼠科啮齿动物（稻鼠）的栖息地范围内。我们在玻利维亚已鉴定出一种来自小稻鼠的基因新型汉坦病毒（马莫雷河病毒[RM]）。描述了小（S）基因组的完整序列和中（M）基因组的部分序列。该病毒与新发现的来自巴塔哥尼亚的人类病原体安第斯病毒密切相关。为便于改进南美洲汉坦病毒感染的诊断，我们在大肠杆菌中表达了RM的完整核衣壳蛋白，并对其进行亲和纯化，用于RM抗体的酶联免疫吸附测定（ELISA）和蛋白质免疫印迹分析。
