Mank T G, Zaat J O, Deelder A M, van Eijk J T, Polderman A M
General Practitioners Laboratory, Haarlem, The Netherlands.
Eur J Clin Microbiol Infect Dis. 1997 Aug;16(8):615-9. doi: 10.1007/BF02447929.
The substitution of enzyme immunoassay (EIA) techniques for microscopy as a screening tool for Giardia lamblia infection was assessed. Paired stool samples obtained within a ten-day period from 366 patients with persistent diarrhea were examined by microscopy. In addition, two commercially available Giardia lamblia-specific EIAs were performed. Compared with microscopy, EIA for copro-antigen detection was more sensitive, based on examination of either one or two stool samples. Repeated examinations increased the number of cases detected, more so for microscopy than EIA. The negative predictive values of the two EIAs performed on the first stool sample were 98.7% and 97.8%. The results show that EIA for detection of copro-antigens in a single stool sample may be almost as sensitive for identifying Giardia infection as repeated microscopy on two sequential stool samples.
评估了用酶免疫测定（EIA）技术替代显微镜检查作为蓝氏贾第鞭毛虫感染筛查工具的情况。对366例持续性腹泻患者在10天内采集的配对粪便样本进行了显微镜检查。此外，还进行了两种市售的蓝氏贾第鞭毛虫特异性EIA检测。与显微镜检查相比，基于对一份或两份粪便样本的检测，用于粪便抗原检测的EIA更敏感。重复检测增加了检测到的病例数，显微镜检查增加的更多，EIA增加的较少。对第一份粪便样本进行的两种EIA的阴性预测值分别为98.7%和97.8%。结果表明，在单一粪便样本中检测粪便抗原的EIA对于识别贾第鞭毛虫感染的敏感性几乎与对两份连续粪便样本进行重复显微镜检查相同。
