鉴定感染美国犬蜱（Dermacentor andersoni）的一种内共生菌为弗朗西斯菌属的成员。
Characterization of an endosymbiont infecting wood ticks, Dermacentor andersoni, as a member of the genus Francisella.
作者信息
Niebylski M L, Peacock M G, Fischer E R, Porcella S F, Schwan T G
机构信息
Laboratory of Microbial Structure and Function, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA.
出版信息
Appl Environ Microbiol. 1997 Oct;63(10):3933-40. doi: 10.1128/aem.63.10.3933-3940.1997.
Abstract
A microorganism (Dermacantor andersoni symbiont [DAS]) infecting Rocky Mountain wood ticks (D. andersoni) collected in the Bitterroot Mountains of western Montana was characterized as an endosymbiont belonging to the genus Francisella. Previously described as Wolbachia like, the organism's DNA was amplified from both naturally infected tick ovarial tissues and Vero cell cultures by PCR assay with primer sets derived from eubacterial 16S ribosomal DNA (rDNA) and Francisella membrane protein genes. The 16S rDNA gene sequence of the DAS was most similar (95.4%) to that of Francisella tularensis subsp. tularensis. Through a combination of Giménez staining, PCR assay, and restriction fragment length polymorphism analysis, 102 of 108 female ticks collected from 1992 to 1996 were infected. Transovarial transmission to female progeny was 95.6%, but we found no evidence of horizontal transmission.
摘要
从蒙大拿州西部比特鲁特山脉采集的感染落基山木蜱（Dermacentor andersoni）的一种微生物（安德森革蜱共生菌 [DAS]）被鉴定为属于弗朗西斯菌属的一种内共生菌。该生物体以前被描述为类似沃尔巴克氏体，通过使用源自真细菌16S核糖体DNA（rDNA）和弗朗西斯菌膜蛋白基因的引物对进行PCR检测，从自然感染的蜱卵巢组织和Vero细胞培养物中扩增出了该生物体的DNA。DAS的16S rDNA基因序列与土拉弗朗西斯菌土拉亚种的序列最为相似（95.4%）。通过吉姆萨染色、PCR检测和限制性片段长度多态性分析相结合的方法，对1992年至1996年采集的108只雌蜱中的102只进行检测，发现它们受到了感染。向雌性后代的经卵传递率为95.6%，但我们没有发现水平传播的证据。
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